Direct Susceptibility Testing on MGIT 960 TB System: a Rapid Method for Detection of Drug Resistant Tuberculosis.

Nadia Tayyab; Gohar Zaman; Luqman Satti; Aamer Ikram; Adeel Hussain Gardezi; Muhammad Tahir Khadim

Author(s)

Nadia Tayyab Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Gohar Zaman Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Luqman Satti Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Aamer Ikram Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Adeel Hussain Gardezi Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Muhammad Tahir Khadim Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan

Abstract

Objective: To evaluate direct drug susceptibility testing on MGIT 960 system for detection of multidrug resistant tuberculosis from smear positive pulmonary specimens. Study Design: Cross-sectional analytical study. Place and Duration of Study: Microbiology Department, Armed Forces Institute of Pathology, Rawalpindi, from July 2016 to September 2017. Methodology: Smear positive specimens were pretreated according to guidelines and then tested on MGIT 960 TB system for direct drug susceptibility testing (DST) of isoniazid and rifampin. Samples were also processed by gold standard indirect method, which comprises culture and then DST from positive growth by MGIT 960 TB system. Results: Out of 108 specimens, 95 (88%) DST results were reportable. Out of 95 reportable specimens, 17 isolates were resistant to both isoniazid (INH) and rifampin (RIF) by direct DST. The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy for INH were 92%, 93%, 82%, 97% and 92.6%, respectively; and 95%, 96%, 86.3%, 98.6% and 95.7%, respectively for RIF. Average time to report DST by indirect method was 23.6 ±3.9 days, while it was 11.4 ±2.7 days for the direct method. Conclusion: Direct susceptibility testing on MGIT 960 system showed very good agreement when compared with indirect method. Time saving is crucial factor in initiation of early effective therapy, especially in drug resistant cases. Further studies on large scale are required for more accurate evaluation of this method.

Publication Details

Page(s) 590-593

DOI DOI not available

Published Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 28, Issue: 8, Year: 2018

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