Optimization, validation and application of quantitative method for the determination of acrylamide in potato chips samples in yemen using GC-NPD

Anass A. Alnedhary; Abdualqawi A. Numan; Mahfoudh M. AL-Hammadi; Taiseer H. Al-Hoded

doi:10.21743/pjaec/2023.06.06

Author(s)

Anass A. Alnedhary Chemistry Department, Faculty of Education, Khawlan Branch, Sana'a University, Sana'a, Yemen.

Abdualqawi A. Numan Taiz University, Faculty of Applied Sciences, Chemistry Department, Taiz, Yemen; Aljanad University for Science and Technology, Faculty of Medical Sciences, Pharmacy Department, Taiz, Yemen.

Mahfoudh M. AL-Hammadi Chemistry Department, Faculty of Science, Sana'a University, Sana'a, Yemen.

Taiseer H. Al-Hoded Chemistry Department, Faculty of Science, Sana'a University, Sana'a, Yemen.

Abstract

Over the last two decades, numerous analytical methods for determining acrylamide concentration in a wide range of food products have been developed. Some studies focused on the methods of extracting acrylamide from complex food matrices. In the present study, solid Phase Extraction (SPE) and Gas Chromatography-Nitrogen Phosphorus Detector (GC-NPD) analysis were employed to develop a simple and cost-effective acrylamide determination method. Method validation and optimization steps were carried out and used to determine acrylamide concentrations in potato chips purchased from Yemeni markets. In the concentration range of 0.1- 5 ppm, the validated method demonstrated good sensitivity within a 0.012 ppm limit of detection (LOD) and an excellent linearity of 0.996 correlation coefficient. Eighteen samples of potato chips purchased from local markets in Sana'a, Yemen, were analyzed using this method. Three of the 18 samples of interest contained various acrylamide concentration levels of 879.4, 795.6, and 754.7 ppb. These were higher than the EU Commission's indicative value for the acrylamide in potato chips (750 ppb). As a result, the optimized GC-NPD protocol proved to be useful for the acrylamide analysis, paving the way for the development of an acrylamide quantification prescreening tool in the food industry.

Publication Details

Page(s) 63-73

DOI 10.21743/pjaec/2023.06.06

Published Journal: Pakistan Journal of Analytical & Environmental Chemistry, Volume: 24, Issue: 1, Year: 2023

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Keywords

Acrylamide, GC/NPD, c-SPE, Potato Chips