To evaluate chromosomal analyses, including chromosomal abnormalities which are the underlying cause of many hereditary illnesses including cancer, mental retardation, and infertility, to name a few cytogenetic investigations depend on widely distributed metaphase chromosomes. Cell geneticists still use the traditional method of chromosomes spread, which may need time to adjust the balance of the drops fall of cell suspension solution accurately on the slides. In addition, traditional method needs an expertise to provide fast and accurate analytical results and to gain a better understanding of the dynamics of chromosomes spread. The main goal of this research is to study the spread of usable chromosomes, considering the height of the drops fall of the cells suspension solution of mice bone marrow from a height of 30, 40, 50, 60 and 70 cm as well as angles of 0 °, 15 °, 30 °, 45 ° and 60 °. The quality of chromosomal analysis was evaluated by classifying it as ideal, expanded or compacted. More optimal and usable spaces were produced at angles of 30 ° or 45 ° degrees, while the best height of the chromosomal spread was recorded at 70 cm. In this study, chromosomes were spread onto glass slides using a novel approach, with the most optimal spread being at an angle of 30° and a height of 70 cm.