Virtual laboratory based on MATLAB GUI to complement the teaching of the course, to help teachers explain the curriculum theory better, and enhance students understanding of the course. In this paper, listed common experiment in "Automatic Control Theory", and given the GUI panel design and program design. Given the corresponding parameter can get curves and data in applications, to further analyze the influence of different parameters on the system. The method has a friendly interface, easy operation, precise and intuitive image data make up for deficiencies in the traditional teaching, help to improve the quality of teaching and student understanding of "Automatic Control Theory" course.