Identification of Differentially Expressed Transcripts in Development during Free Nuclear Mitosis in Pinus tabulaeformis Ovule.

Yang Yao; Binli Chen; Jun An; Caixia Zheng

doi:10.17957/IJAB/15.0651

Author(s)

Yang Yao College of Biological Sciences and Technology, Beijing Forestry University, No.35, Rd. Qinghuadonglu, Haidian Dist, Beijing 100083, China

Binli Chen College of Biological Sciences and Technology, Beijing Forestry University, No.35, Rd. Qinghuadonglu, Haidian Dist, Beijing 100083, China

Jun An College of Biological Sciences and Technology, Beijing Forestry University, No.35, Rd. Qinghuadonglu, Haidian Dist, Beijing 100083, China

Caixia Zheng College of Biological Sciences and Technology, Beijing Forestry University, No.35, Rd. Qinghuadonglu, Haidian Dist, Beijing 100083, China

Abstract

Ovule development is an important step in the life cycle of sexually reproducing organism. In Pinus tabulaeformis, within the ovule, the female gametophyte undergoes dozens of syncytial mitotic divisions, followed by cellularization to form archegonium. However, at the stage of free nuclear mitosis of female gametophyte, the molecular mechanism is still unknown. In the present study, suppression subtractive hybridization (SSH) was used to identify rare and differentially expressed transcripts in developing (fertile clone, FC) and aborted (sterile clone, SC) P. tabulaeformis ovules. Both forward and reverse SSH libraries were constructed, and756 clones were randomly selected from the libraries and sequenced, yielding valid expressed sequenced tags (ESTs) ranging from 105 bp to 763 bp. Out of them, 233 ESTs from FC and 197 ESTs from SC SSH libraries showed high similarity to BLAST hits, and 336 (47.9%) proteins were annotated to gene ontology (GO) terms. The putative functions of differentially expressed transcripts were involved in most aspects of biological processes. The reliability of SSH libraries and expression patterns of 10 differentially expressed transcripts were confirmed by real-time quantitative PCR. We identified hundreds of differentially expressed transcripts mainly involved in cellular processes, other metabolism, developmental processes and protein metabolism. Furthermore, 27 candidate genes were focused, which might be responsible for mitotic progression, phytohormone regulation and defense response during ovule development. Taken together, this study provides transcriptional information on ovule development in P. tabulaeformis, offers a group of potential developmental-related regulators and poses hypothesis for the abortion of P. tabulaeformis ovules.

Publication Details

Page(s) 1479-1487

DOI 10.17957/IJAB/15.0651

Published Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 7, Year: 2018

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