No Detail Found!

Transcriptome-guided Gene Isolation, Characterization and Expression Analysis of a Phosphomevalonate Kinase Gene (GBPMK) from Ginkgo Biloba.

Author(s)
Qiling Song College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Xiangxiang Meng College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Yongling Liao College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Weiwei Zhang College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Jiabao Ye College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Feng Xu College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
Abstract
Terpenoids are main active ingredients of Ginkgo biloba. Phosphomethoxylate kinase (PMK) is one of the core enzyme in the mevalonate pathway, one of the two pathways in plants that can synthase terpenoids. In this study, a novel PMK gene (designated as GbPMK) was cloned from G. biloba. The expression profile of GbPMK in different tissues (roots, stems, leaves, male strobili, female strobili, and fruits) and under the treatments of hormones and stresses were investigated by qRTPCR. Bioinformatics analysis indicated that the cDNA sequence of GbPMK contained a 1557-bp open reading frame encoding 519 amino acids. Protein structure analysis showed that the GbPMK protein had four conserved domains and one conserved region of the GHMP kinase family. Phylogenetic tree analysis revealed that GbPMK possessed a conserved sequence structure and sequence characteristics. The qRT-PCR analysis results showed that GbPMK exhibited tissue-specific expression, with the highest expression in leaves and the lowest expression in male strobili. GbPMK exerted different degrees of response to treatments with MeJA, ABA, Eth, SA, dark and low temperature but did not respond to wound treatment. The characterization and expression analysis of GbPMK contributed to understanding the biosynthesis of terpenoids at the molecular level. This study provides molecular information for the future through genetic engineering techniques to improve the yield of terpenoids in G. biloba.
Publication Details
Page(s) 1080-1088
Published Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 5, Year: 2018

Request the full text from PASTIC

References
And , C.F.,J.Chory, 1997.Light control of plant development,Annu. Rev. Cell Dev 13 203 -229
Bingham , I.J. and V.B.,Mccabe, 2006.Commercially available plant growth regulators and promoters modify bulk tissue abscisic acid concentrations in spring barley, but not root growth and yield response to drought, 149 291 -304
Boonsri , P.,T.S.Neumann,A.L.Olson,S.Cai,T.J.Herdendorf,H.M.Miziorko,S.Hannongbua,D.S.Sem, 2013.Molecular docking and NMR binding studies to identify novel inhibitors of human phosphomevalonate kinase,Biochem. Biophys. Res. Commun. 430 313 -319
Chen , Q.,J.Yan,X.Meng,F.Xu,W.Zhang,Y.Liao,J.Qu, 2017.Molecular cloning, characterization, and functional analysis of Acetyl-CoA C-Acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from Ginkgo biloba,Molecules 22 74 -90
Conrath , U.,C.M.J. Pieterse and B.,Mauch-Mani, 2002.Priming in plantpathogen interactions,Trends Plant Sci. 7 210 -216
Endt , D.V.,M.S.E.Silva,J.W.Kijne,G.Pasquali,J.Memelink, 2007.Identification of a bipartite jasmonate-responsive promoter element in the Catharanthus roseus ORCA3 transcription factor gene that interacts specifically with at-hook dna-binding proteins,Plant 144 1680 -1689
Gao , S.,J.Lin,X.Liu,Z.Deng,Y.Li,X.Sun,K.Tang, 2006.Molecular cloning, characterization and functional analysis of a 2Cmethyl- D-erythritol 2, 4-cyclodiphosphate synthase gene from Ginkgo biloba,J. Biochem. Mol. Biol 39 502 -510
Gharib , F.A., 2007.Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram,Int. J. Agric. Biol 4 485 -492
Guo , P.,W.Shi,L.Li,Y.Bao, 2018.a protein phosphatase 2C gene of alfalfa, confers enhanced salt-tolerance in Arabidopsis,Int. J. Agric. Biol 20 62 -70
Han , L.,L.B.Han,L.X.Xu, 2013.Effects of ethephon treatment on plant drought tolerance(review),Acta Agrestia Sin 21 631 -636
Herdendorf , T.J. and H.M.,Miziorko, 2006.Phosphomevalonate kinase: functional investigation of the recombinant human enzyme,Biochemistry 45 3235 -3242
Huang , M.,A.M. Sanchez , C.,Abel , R.,Sohrabi , S.,Lee , J.,Gershenzon and D.,Tholl, 2012.The major volatile organic compound emitted from Arabidopsis thaliana flowers, the sesquiterpene (E)-β-caryophyllene, is a defense against a bacterial pathogen, 193 997 -1008
Ji , H.C.,Z.X.Su,J.Yang,C.X.Bian,J.Y.Hu,G.Qi, 2010.Cloning and expression of a cold-induced gene (DiRCI) from Davidia involucrata (Davidiaceae),Acta Bot. Yunnanica 32 151 -157
Ketchum , R.E.,D.M.Gibson,R.B.Croteau,M.L.Shuler, 2015.The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate,Biotechnol 62 97 -105
Ketchum , R.E.,M.Tandon,D.M.Gibson,T.Begley,M.L.Shuler, 1999.Isolation of labeled 9-dihydrobaccatin III and related taxoids from cell cultures of taxuscell cultures of taxus Canadensis elicited with methyl jasmonate,J. Nat. Prod 62 1395 -1398
Kim , J.H.,K.I.Lee,Y.J.Chang,S.U.Kim, 2012.Developmental pattern of Ginkgo biloba levopimaradiene synthase (GbLPS) as probed by promoter analysis in Arabidopsis thaliana,Plant Cell Rep 31 1119 -1127
Kim , S.M.,T.Kuzuyama,Y.J.Chang,S.U.Kim,D, 2006.-erythritol 2,4-cyclodiphosphate synthase (MECS) gene from Ginkgo biloba,Plant Cell Rep 25 829 -835
Lange , B.M.,T.Rujan,W.Martin,R.Croteau, 2000.Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes,Proc. Natl. Acad. Sci. USA 97 13172 -13177
Laule , O.,A.Fürholz,H.S.Chang,T.Zhu,X.Wang,P.B.Heifetz,W.Gruissem,M.Lange, 2003.Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana,Proc. Natl. Acad. Sci. USA 100 6866 -6871
Lee , S.K.,H.Chou,T.S.Ham,T.S.Lee,J.D.Keasling, 2008.Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels,Curr. Opin. Biotechnol 19 556 -563
Liao , Y.,F.Xu,X.Huang,W.Zhang,X.Wang,Y.Shen, .Characterization and transcriptional profiling of Ginkgo biloba mevalonate diphosphate decarboxylase gene (GbMVD) promoter towards light and exogenous hormone treatments,Plant Mol. Biol. Rep 34 1 -16
Liao , Y.,F.Xu,X.Huang,W.Zhang,Li , S. Cheng and Y.,Shen, .Promoter analysis and transcriptional profiling of Ginkgo biloba 3-hydroxy-3-methylglutaryl coenzyme a reductase (GbHMGR) gene in abiotic stress responses,Not 43 25 -34
Liao , Y.L.,F.Xu,W.W.Zhang,Y.B.Shen,J.Chang, 2016.Cloning, characterization and expression analysis of gbwrky11, a novel transcription factor gene in Ginkgo biloba,Int. J. Agric. Biol 18 117 -124
Mou , L.L., 2008.Antioxidant activities of the chemical constituents isolated from the leaves of Ginkgo biloba,Chin. J. Nat. Med 6 26 -29
Lombard , J. and D.,Moreira, 2011.Origins and early evolution of the mevalonate pathway of isoprenoid biosynthesis in the three domains of life,Mol. Biol 28 87 -99
Miziorko , H.M., 2011.Enzymes of the mevalonate pathway of isoprenoid biosynthesis,Arch. Biochem. Biophys. 505 131 -143
Morgan , P.W. and H.W.,Gausman, 1966.Effects of ethylene on auxin transport,Plant 41 45 -52
Pang , Y.,G.A.Shen,T.Bergès,H.Cardier,W.Wu,X.Sun,K.Tang, 2006.Molecular cloning, characterization and heterologous expression in Saccharomyces cerevisiae of a mevalonate diphosphate decarboxylase cDNA from Ginkgo biloba,Physiol. Plant 127 19 -27
Parveen , I.,M.Wang,J.Zhao,A.G.Chittiboyina,N.Tabanca,A.Ali,S.R.Baerson,N.Techen,J.Chappell,I.A.Khan, 2015.Investigating sesquiterpene biosynthesis in Ginkgo biloba: molecular cloning and functional characterization of (E, E)-farnesol and α-bisabolene synthases,Mol. Biol 89 451 -462
Peraltayahya , P.P.,M. Ouellet , R.,Chan , A.,Mukhopadhyay , J.D.,Keasling and T.S.,Lee, 2011.Identification and microbial production of a terpene-based advanced biofuel,Nat. Commun 2 483 -491
Piccoli , P.,R.Bottini, 2013.Terpene production by bacteria and its involvement in plant growth promotion, stress alleviation, and yield increase,Mol. Microbiol 65 335 -343
Pichersky , E.,J.Gershenzon, 2002.The formation and function of plant volatiles: perfumes for pollinator attraction and defense,Curr. Opin. Plant Biol 5 237 -243
Pilloff , D.,K.Dabovic,M.J.Romanowski,J.B.Bonanno,M.Doherty,S.K.Burley,T.S.Leyh, 2003.The kinetic mechanism of phosphomevalonate kinase,J. Biol. Chem 278 4510 -4515
Robertseilaniantz , A.,M.Grant,J.D.Jones, 2011.Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism,Annu. Rev. Phytopathol. 49 317 -343
Schmittgen , T.D. and K.J.,Livak, 2008.Analyzing real-time PCR data by the comparative CT method,Nat. Protoc 3 1101 -1108
Schnee , C.,T.G.Köllner,M.Held,T.C.J.Turlings,J.Gershenzon,J.Degenhardt, 2006.The products of a single maize sesquiterpene synthase form a volatile defense signal that attracts natural enemies of maize herbivores,Proc. Natl. Acad. Sci. USA 103 1129 -1134
Schwarz , M.,D.Arigoni, 1999.,Compr. Nat. Prod. Chem 2 367 -400
Shen , G.,Y.Pang,W.Wu,Z.Liao,L.Zhao,X.Sun,K.Tang, 2006.Cloning and characterization of a root-specific expressing gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Ginkgo biloba,Mol. Biol. Rep 33 117 -127
Singh , P.,T.S.Batth,D.Juminaga,R.H.Dahl,J.D.Keasling,P.D.Adams,C.J.Petzold, 2012.Application of targeted proteomics to metabolically engineered Escherichia coli,Proteomics 12 1289 -1299
Smith , J.V.,Y.Luo, 2004.Studies on molecular mechanisms of Ginkgo biloba extract,Appl 64 465 -472
Strømgaard , K.,K.Nakanishi, 2004.Chemistry and biology of terpene trilactones from Ginkgo biloba,Angew. Chem 35 1640 -1658
Thomashow , M.F., 1990.Molecular genetics of cold acclimation in higher plants,Adv. Genet 28 99 -131
Thulasiram , H.V.,H.K.Erickson,C.D.Poulter, 2007.Chimeras of two isoprenoid synthases catalyze all four coupling reactions in isoprenoid biosynthesis,Science 316 73 -76
Vranová , E.,D.Coman,W.Gruissem, 2013.Network analysis of the MVA and MEP pathways for Isoprenoid synthesis,Annu. Rev. Plant Biol 64 665 -700
Wang , L.,X.Fang,C.Yang,J.Li,X.Chen, 2013.Biosynthesis and regulation of secondary terpenoid metabolism in plants,Sci. Sin 43 1030 -1046
Wang , P.,Z.Liao,L.Guo,W.Li,M.Chen,Y.Pi,Y.Gong,X.Sun,K.Tang, 2004.Cloning and functional analysis of a cDNA encoding Ginkgo biloba farnesyl diphosphate synthase,Mol. Cells 18 150 -156
Xiao , W.,H.Chang,P.Zhou,C.Yuan,C.Zhang,R.Yao,X.Lu,L.Cai,Z.He,R.Hu, 2015.Genome-wide identification, classification and expression analysis of GHMP genes family in Arabidopsis thaliana,Plant Syst. Evol 301 2125 -2140
Yang , Z.,L.Tian,M.Latoszekgreen,D., 2005.Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses,Mol. Biol 58 585 -596
Yonekura-Sakakibara , K.,K.Saito, 2009.Functional genomics for natural product biosynthesis,Nat. Prod. Rep 26 1466 -1487
Yu , F.,R.Utsumi, 2009.genetic manipulation of plant mono- and sesquiterpenoid biosynthesis,Cell. Mol. Life Sci 66 3043 -3052
Yuan , C.,H.Chang,J.Z.Ye,Y.Wang,S.X.Hu,H.B.Hu,X.S.Li,X.Guo, 2012.Tissue expressions and bioinformatic analysis of GHMP gene family members in Arabidopsis,Acta Laser Biol 21 346 -351
Yuan , Y.,J.Yu,C.Jiang,M.Li,S.Lin,X.Wang,L.Huang, 2013.Functional diversity of genes for the biosynthesis of paeoniflorin and its derivatives in Paeonia,Int. J. Mol. Sci 14 18502 -18519
Zeng , Z.,J.Zhu,L.Chen,W.Wei,R.Yu, 2013.Biosynthesis pathways of ginkgolides,Pharmacogn. Rev. 7 47 -52
0

Citations

0

Downloads

52

Views