Objective: To identify the frequency of Pseudomonas aeruginosa and its sensitivity to various antibiotics in patients of chronic suppurative otitis media. Material and Methods: This descriptive study was conducted at the Department of ENT, DHQ Teaching Hospital, Dera Ismail Khan from January 2011 to October 2011. Patients with unilateral or bilateral active chronic suppurative otitis media attending the out patient clinic were included in the study. All the patients were evaluated through detailed history and clinical examination. Pus samples were collected from the discharging ears and sent to the Microbiology Department, Combined Military Hospital, Dera Ismail Khan for culture and sensitivity studies. Results: From the clinical specimens of 220 patients enrolled in the study, microbiological culture was yielded from 198(90%) specimens. Out of total 198 cultured isolates, Pseudomonas aeruginosa was isolated in 96/198(43.65%) cases. Drug sensitivities pattern showed that tazocin (piperacillin/tazobactum) had highest sensitivity 96 (100%) while gentamicin had lowest sensitivity 48 (50%) against Pseudomonas aeruginosa isolates. Conclusion: Pseudomonas aeruginosa was the commonest isolate from chronic discharging ears. Pseudomonas aeruginosa was 100% sensitive to tazobactem-piperacillin. Pseudomonas aeruginosa is increasingly becoming more resistant to the common drugs like quinolones.