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Orthogonal experimental design on ethanol production from kitchen garbage using acid-tolerant bacteria under non-sterilized fermentation.

Author(s)
Wenyu Zhang Department of Environmental Engineering, University of Science and Technology, Beijing, Beijing, 100083, China
Hongzhi Ma Department of Environmental Engineering, University of Science and Technology, Beijing, Beijing, 100083, China
Qunhui Wang Department of Environmental Engineering, University of Science and Technology, Beijing, Beijing, 100083, China
Abstract
To produce ethanol from kitchen garbage under an acidic condition instead of a sterilized condition, a strain of acid-tolerant Zymomonas mobilis, GZNS1, was used for ethanol fermentation in the present study. Thermal gravity (TG) and differential scanning calorimetry(DSC) analysis were carried out to evaluate the characteristic of kitchen garbage. An orthogonal experimental design was adopted to determine the optimum conditions in consideration of ethanol. TG and DSC result showed that the gelatinization heat of the kitchen garbage was much lower than those from other materials, which meant this was a suitable substrate for un-cooked fermentation. The isolated GZNS1 could produce ethanol efficiently at pH 4 without sterilization. Maximum ethanol concentration of 53 g/L was obtained under optimal conditions of 40 h fermentation time, pH 4, temperature of 29 °C, and 9% (V/M) inoculum size. Ethanol fermentation from kitchen garbage under an acidic condition using an acid-tolerant strain without sterilization is proved feasible. This low-cost and simple technology should be studied further for industrial applications.
Publication Details
Page(s) 1218-1223
DOI DOI not available
Published Journal: Journal of Chemical Society of Pakistan, Volume: 34, Issue: 5, Year: 2012

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