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In vitro mutagenesis in sugarcane.

Author(s)
Imtiaz Ahmed Khan Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Abdullah Khatri Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Maqbool Ahmad Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Shamim Hussain Siddiqui Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Ghulam Shah Nizamani Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Mohammad Hussain Khanzada Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Nazir Ahmed Dahar Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Raziullah Khan Atomic Energy Agricultural Research Centre, Tandojam, Pakistan
Abstract
Two sugarcane clones viz., AEC81-8415 and BL4 were tested for in vitro mutagenesis using irradiation doses of 0, 10, 20, 30, 40 and 50Gy. Maximum callus proliferation and shoot regeneration was observed in 10Gy in both clones with linear decrease in callusing and regeneration potential when the irradiation dose was increased upto 50Gy. Better response in root development was observed in MS medium containing 6% sucrose and 1mg/IBA. Data on quantitative and qualitative traits were also recorded.
Publication Details
Page(s) 253-261
DOI DOI not available
Published Journal: Pakistan Journal of Botany, Volume: 30, Issue: 2, Year: 1998

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