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Solid-state fermentation of pullulanase by Bacillus subtilis.

Author(s)
J. Iqbal Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore, Pakistan
R. Ahmad Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore, Pakistan
S. A. Nagra Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore, Pakistan
M. A. Qadeer Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore, Pakistan
Abstract
The production of enzyme pullulanase by Bacillus subtilis M-21 was studied by solid substrate fermentation. The agricultural by-products such as wheat bran, rice bran, soybean meal, sunflower meal or corn meal were evaluated. Phosphate buffer (pH 7.0) was used to moisten the substrate and the enzyme was extracted using acetate buffer (PH-6.2). Wheat bran provided all necessary ingredients for propagation of the culture and enzyme formation. Partial replacement of wheat bran with other substrates did not show any significant effect on enzyme formation. The thickness of bran layer of 0.8 cm in one L conical flask gave better results of enzyme synthesis. The fermentation was reached maximum, 48 hours after inoculation. Effect of the addition of different carbon and nitrogen sources was also investigated.
Publication Details
Page(s) 17-21
DOI DOI not available
Published Journal: Pakistan journal of scientific research, Volume: 49, Issue: 1--2, Year: 1997

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