Different loss assessment methods were tested on wheat in the laboratory. These methods included Standard Volume Weight (SVW), Thousand Grain Mass (TGM), Multiple Thousand Grain Mass (M. TGM), Count and Weigh (CW), Multiple Count and Weigh (M. CW) and Indirect (IND). These methods were compared with a Standard Method (STD) in which each replicate was Weighed after sieving at monthly interval. There were no significant differences in all these methods after 1st and 2nd month of storage. In 3rd month STD and SVW were significantly different from others. In 4th and 5th months, STD M. TGM, and TGM were significantly different from other methods. Highest correlation was exhibited between STD and sieved material (t=0.85) and next in line was between sieved material and indirect method (t=0.62). Also highest correlation between IND and living insects was found (t=0.59). Results showed that if wheat has been stored for a longer period the IND gave results which are nearest to the STD and can be considered more reliable than other methods as correlation coefficient in this method is nearest to STD.