Strawberry (Fragaria ananassa Duch cv. 'Red Gauntlet'), plantlets produced in vitro, were subjected to rooting both in vitro and in vivo. In vitro rooting was maximum on inorganic salts of Murashige and Skoog medium augumented with 2% sucrose. Actively proliferating meristems, maintained on BA enriched shoot culture medium in vitro, produced roots easily than the meristems taken from 60-day-old cultures. All the in vitro produced plantlets rooted easily in different type of soils, while only 25% actively proliferating meristems could produce roots in the soil. In the greenhouse, for in vivo survival and growth of in vitro propagated strawberry plantlets, mist was necessary for first seven days.