40x60x10 healthy spores of penicillin culture are introduced into seed vessel containing sterilized media where it is run till the mycelia is about 5% (40-50) hours. It is, then, transferred to fermenter containing sterilized media into which sugar, oil and precursor fed during the run. During fermentation many penicilin are produced showing antibacterial activity such as pencillin X, Phonethecillin, Penicillin V, Phenoxy prop Penicillin, Methicillin, Penbriton, Oxacillin, Cloxacill Dicloxacillin, Nafcillin, Hetacillin, Pyopen and Residual Penicillin.