Glucoamylase production from Aspergillus niger by using solid state fermentation process.

Muhammad Imran; Muhammad Javaid Asad; Muhammad Gulfraz; Hina Gul; Nazish Manzoor; Rahmatullah Qureshi; Arshad Nawaz Choudhary

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Glucoamylase production from Aspergillus niger by using solid state fermentation process.

Author(s)

Muhammad Imran Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Muhammad Javaid Asad Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Muhammad Gulfraz Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Hina Gul Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Nazish Manzoor Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Rahmatullah Qureshi Department of Botany, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Arshad Nawaz Choudhary Department of Soil Sciences, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan

Abstract

The main objective of this study was to produce glucoamylase under optimum conditions and to study the effect of chemical mutagenesis on Aspergillus niger for the production of glucoamylase. The maximum activity of glucoamylase (3.185±0.020 IU/mL/min.) by mutant Aspergillus niger and (2.085±0.021 IU/mL/min.) for wild Aspergillus niger was recorded in the culture filtration after 96 hours of Solid State Fermentation of growth medium with 70% moisture level and in presence of 0.3% yeast extract, 0.4% peptone, and 4 mL Tween-80 at pH 4.8. The maximum fraction value after gel filtration for wild Aspergillus niger was 2.850 IU/mL/min and for mutant Aspergillus niger was 2.980 IU/mL/min. Purification through the SDS PAGE revealed the indication of glucoamylase purification from Aspergillus niger. The high value of Km shows that substrate had great affinity for glucoamylase. Glucoamylase enzyme has many useful applications in food processing industry and fermentation biotechnology.

Publication Details

Page(s) 2103-2110

DOI DOI not available

Published Journal: Pakistan Journal of Botany, Volume: 44, Issue: 6, Year: 2012

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