Fingerprinting for discriminating tea germplasm using Inter-Simple Sequence Repeat (ISSR) markers .

B. Y. Liu; H. Cheng; Y. Y. Li; L.Y. Wang; W. He; P. S. Wang

B. Y. Liu Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai Yunnan 666201, P. R. China

H. Cheng Research Center for Tea Germplasm Breeding and Improvement, Tea Research Institute of Chinese Academy of Agricultural Science, National Center for Tea improvement, Hangzhou Zhejiang 310008, P. R. China

Y. Y. Li Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai Yunnan 666201, P. R. China

L.Y. Wang Research Center for Tea Germplasm Breeding and Improvement, Tea Research Institute of Chinese Academy of Agricultural Science, National Center for Tea improvement, Hangzhou Zhejiang 310008, P. R. China

W. He Research Center for Tea Germplasm Breeding and Improvement, Tea Research Institute of Chinese Academy of Agricultural Science, National Center for Tea improvement, Hangzhou Zhejiang 310008, P. R. China

P. S. Wang Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai Yunnan 666201, P. R. China

For the discrimination of tea germplasm at the inter-specific level, 134 tea varieties preserved in the China National Germplasm Tea Repositories (CNGTR) were analyzed using inter simple sequence repeat (ISSR) markers. Eighteen primers were chosen from 60 screened for ISSR amplification, generating 99.4% polymorphic bands. The mean Nei’s gene diversity (H) and the overall mean Shannon’s Information index (I) were 0.396 and 0.578, respectively, indicating a wide gene pool. Using the presence, sometimes absence of unique ISSR markers, it was possible to discriminate 32 of the genotypes tested. No single primer could discriminate all the 134 genotypes. However, UBC811 provided rich band patterns and it can discriminate 35 genotypes. The combination of two and three primers could discriminate 99 and 121 genotypes, respectively. Furthermore, the combination of band patterns or the DNA fingerprinting based on specific ISSR markers generated by UBC811, UBC835, ISSR2 and ISSR3 could discriminate all 134 genotypes tested. ISSR markers also provide a powerful tool to discriminate tea germplasm at the inter-specific level.