Somatotropin (ST), an essential polypeptide required for normal growth and development of vertebrates, is commercially important for a variety of applications. Despite the high degree of evolutionary conservation amongst the avian STs, significant variations in sequence at nucleotide and/or amino acid levels have been reported. The present study describes sequence and expression of ST gene of an indigenous chicken (Gallus gallus) breed `Aseel` (GgST-A), one of the oldest known cock-fighting bird, in Escherichia coli. Total RNA Extracted from the pituitary glands of Aseel was used as template for RT-PCR based amplification of -600 by ST cDNA. The ampl icon was cloned in pTZ57RJT vector and subjected to sequence analysis. Four sequence variations at the nucleotide level were observed, when the GgST-A sequence was compared with the ST sequence of G. gallus domesticus (Desi breed). Amongst these, one point mutation led to F-*Y amino acid change at position 184. The GgST-A cDNA was also subcloned in pET-22b (+) vector and its expression was analyzed in E. coli strain BL21 (DE3) CodonPlus. High-level expression of GgST-A corresponding to >30 % of the total E. coli cell proteins was achieved. Recombinant protein Expression was however, in the form of inclusion bodies (I Bs), which were solubilized using mi Id concentration of urea (2M) under alkaline conditions (pH 12.5) and refolded by dilution and step-dialysis method. In conclusion, the study contributes new sequencing data and highlights the need for protein structural analysis for better insight of the functional and application aspects of GgST-A.