Partial purification and characterization of a xylanase from trichoderma Harzianum

Aamir Abbas; Zahid Mushtaq; Amer Jamil; Sibtain Ahmad

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Partial purification and characterization of a xylanase from trichoderma Harzianum

Author(s)

Aamir Abbas Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Zahid Mushtaq Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Amer Jamil Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Sibtain Ahmad University of New Mexico, MSC10-5550, Albuquerque, NM 87131-000 USA

Abstract

Among the fungi, the soft rot fungus Trichoderma has shown to be efficient producers of xylan degrading enzyme. In this paper we report partial purification and biochemical characterization of a xylanase (XYN) from a local isolate of a filamentous fungus Trichoderma harzianum. Xylanase was produced by growing the T. harzianum in Vogel’s medium where 1 % xylan was used as carbon source in medium to induce the production of enzyme. During purification 40% ammonium sulphate saturation was found to be optimal for xylanase precipitation. The crude sample was dialysed and was also followed by Sephadex G-200 chromatography steps. The specific activity of xylanase was increased about 3.14-fold compared with the crude preparations. The pH and temperature optima of the partiallypurified xylanase was 6.0 and 65 oC respectively. The apparent Km and Vmax values of the crude xylanase using birchwood xylan as a substrate were 1.52 mg mL-1 and 0.647 µmol min-1 mg1 ,respectively.

Publication Details

Page(s) 1455-1459

DOI DOI not available

Published Journal: Journal of Chemical Society of Pakistan, Volume: 34, Issue: 6, Year: 2012

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