Relationships between dnaja1 expression and beef tenderness: effects of electrical stimulation and post-mortem aging in two muscles.

Ahmed  Sami; Edward  Mills; Jean François  Hocquette

Author(s)

Ahmed Sami Department of Animal Production, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt ;Graduate Studies Deanship, King Saud University, Saudi Arabia

Edward Mills The Pennsylvania State University, Department of Animal Science, University Park, USA

Jean François Hocquette INRA, UMR1213, Recherches sur les Herbivores, F-63122 Saint Genès Champanelle, France ;Clermont Université, Vet Agro Sup, UMR1213, Recherches sur les Herbivores, F-63122 Saint Genès Champanelle, France

Abstract

Previous studies have demonstrated that DNAJA1 could be a good indicator of beef toughness in Longissimus thoracis muscle from Charolais young bulls. This study aimed to check if relationship between DNAJA1 expression and beef tenderness is valid in two muscles, which differ in toughness from Angus steers with or without electric stimulation at different times of post-mortem ageing. Electric stimulation did not significantly affect DNAJA1 expression at the time of slaughter. But, RNA degradation soon after slaughter was a major factor, which affected the assessment of DNAJA1 expression, even at 3 h post-mortem. Correlation of DNAJA1 expression at slaughter with shear force values varied according to ageing time (24 h, 3, 7, 14 or 21 days post-mortem) or according to the use of electric stimulation and was not significantly correlated with any of these shear force peak values despite a few high correlation values (-0.60 or +0.34). In conclusion, DNAJA1 is not an omnipotent marker of beef toughness with this particular data set since the correlation of its expression level with shear force values varies according to slaughtering conditions and ageing time.

Publication Details

Page(s) 815-820

DOI DOI not available

Published Journal: International Journal of Agriculture and Biology, Volume: 17, Issue: 4, Year: 2015

Request the full text from PASTIC