Objective: To compare the difference in sagittal cephalometric analysis in centric occlusion (CO) and in centric relation (CR), among Angle’s classes i.e. class I, class II, and class III. Design: Comparative, cross-sectional study. Place and Duration of Study: Orthodontic Outpatient Department, Karachi Medical and Dental College, Karachi, from July 2003 to December 2004. Patients and Methods: Eighty pre-treatment orthodontic patients were divided into 3 groups according to Angle’s classification. The first lateral cephalogram was taken in CO. Then leaf gauges were placed in between incisors for deprogramming. The second cephalogram was taken with leaf gauges in place to obtain centric relation. Both the radiographs were traced. Angle ANB was measured for sagittal analysis. ANB angle compares the position of mandible with the maxilla relating it with the cranial base. Angles from both cephalograms were compared with each other. Results: Significant difference (p