Tissue culture experiment was carried out to study some biochemical changes and molecular aspects of iron deficiency stress in four bread wheat (Triticum aestivum) genotypes. Wheat embryos were grown for 30 days on LS media supplemented with 1x104 (Fe-sufficient) and 0.0 (Fe-deficient) Fe-EDTA, respectively. After 30 days from the beginning of the treatments, it was found that the genotypic difference in growth rate measurement can be related to iron stress, since Callus growth rate was reduced under iron deficient treatments among wheat genotypes. Under Fe-deficiency, a marked reduction (25%) in the growth rate was observed in Sakha 69; whereas, the values of Sakha 61 genotype gave the highest growth rate (100%) under control treatment compared with other genotypes. The specific activity of ascorbate peroxidase (APX) was measured and showed a close relationship with Fe supply and thus a differential sensitivity of wheat genotypes to Fe deficiency. Significant differences were observed between genotypes in APX at 10 and 30 days; whereas, nitrate reductase (NR) showed in wheat genotypes to Fe deficiency. For example at 20 days growth, NR in all genotypes reached optimum level then gradually declined at 30 days growth. It was confirmed that cultivar differences in protein polymorphism could be revealed by electrophoretic patterns. One major protein band with high intensity was induced in all genotype of 26.6 kD in both treatments. However, differences in the protein pattern detected by SDS-PAGE between (Fe-sufficient) and (Fe-deficient) indicated that Fe deficiency led to an alteration in the pattern of proteins synthesis.