The most essential principle in the modern molecular biology is extraction of DNA with a desirable quantity and quality, and this achievement and complete DNA sequencing of genome could be the main necessity for every genetic study till 2010. DNA extraction is obviously difficult because of negative effects obtained- from carbohydrates, tannins, polyphenols and proteins. Therefore, the ideal method is the one, which highly reduces the presence of these materials. In the present study, four special methods are chosen to be compared: (1) Murray and Thompson (1980), (2) Dellaporta et al. (1983), (3) Doyle and Doyle (1990), and (4) Lodhi et al. (1994). Both young and mature leaves of Iranian cyclamen are used in the procedure. The quality and quantity of extracted genomic DNA gained from these methods are deliberated by means of spectrophotometry, electrophoresis in 1% agarose gel and PCR. In this regard, application of Murray-and Thompson`s method (1980) is-chosen for young and mature leaves. The most value of qualified DNA, is extracted from young leaf tissue by 395 ng/Lit and by the same method it -shows a significant difference with the elderly leaves by 355 ng/µL. According to the data, Murray and Thompson`s Method (1980) is recommended for DNA extraction from young leaves of Iranian cyclamen.