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Efficient plant regeneration through somatic embryogenesis from leaf base-derived callus of Kaempferia galanga L.

Author(s)
M. M. Rahman Department of Botany, University of Rajshahi, Rajshahi-5, Bangladesh
M. N. Amin Department of Botany, University of Rajshahi, Rajshahi-5, Bangladesh
T. Ahamed Department of Botany, University of Rajshahi, Rajshahi-5, Bangladesh
M. R. Ali Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
A. Habib Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
Abstract
Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L"`2, 4-D+1.0 mg L-` BA. After transfer on MS medium supplemented with 2.0 mg L-`BA+0.1 mg L­`NAA, this callus produced small globular embryos first that later developed into plantlets by further sub culturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.
Publication Details
Page(s) 675-678
DOI DOI not available
Published Journal: Asian Journal of Plant Sciences, Volume: 3, Issue: 6, Year: 2004

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