Levofloxacin is (-)-(S)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl1-pipe-razinyl)7oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid hemihydrate and a fluorinated quinolone antibacterial agent extensively used against both Gram-positive as well as Gram-negative microorganisms. The in vitro release of Levofloxacin has been studied in presence of metal ions like magnesium, calcium, chromium, manganese, ferric, ferrous, cobalt, nickel, copper, zinc and cadmium in simulated gastric juice, simulated intestinal juice and at blood pH. These studies were carried out using BP 2002 dissolution test apparatus at 37` C. The availability of Levofloxacin was found to be markedly retarded in the presence of all the metals studied.