Passive Immunization against hydropericardium syndrome infected broilers.

G.  Khalid; K.  Muhammad; M.  Rabbani; A.  Ahmed; S. A.  Khan

No Detail Found!

Passive Immunization against hydropericardium syndrome infected broilers.

Author(s)

G. Khalid Department of Microbiology, University of Veterinary and Animal Science Lahore, Pakistan

K. Muhammad Department of Microbiology, University of Veterinary and Animal Science Lahore, Pakistan

M. Rabbani Department of Microbiology, University of Veterinary and Animal Science Lahore, Pakistan

A. Ahmed Department of Microbiology, University of Veterinary and Animal Science Lahore, Pakistan

S. A. Khan Department of Pathology, University of Veterinary & Animal Sciences, Lahore, Pakistan

Abstract

Passive immunization against hydropericardium syndrome virus (HPSV) infected broilers chicks was studied. The hyperimmune yolk and serum were raised in commercial layers by priming and boosting with inactivated HPSV vaccine. The yolk and serum collected from the layers showed high titer of indirect hemagglulination (IHA) antibodies against HPS Virus. Lethal dose 50 (LD50) of the HPS virus infected liver IHA-anti-HPSV antibodies induced 100 percent protection in broiler chicks (non-HPS vaccinated: 26 days old) to challenge infection (I/M injection: 1000 units LD 50 of virulent HPS virus). Yolk and serum containing undetectable level of anti-HPSV-IHA antibodies did not induce protection in the broilers to challenge infection. The immune yolk (1 ml, 64 units of anti HPSV-IHA antibodies) showed 100% protection in birds receiving challenge infection 24 hours before, at the same time or after 24 hours of passive immunization. The immune yolk (when injected 48 hours post challenge infection) induced 60 percent protection in apparently healthy birds and 20 percent protection in the birds showing signs of the disease. From the results, it is concluded that the hyperimmune yolk if administered immediately after the onset of HPS signs in broiler flocks, may mitigate the mortality.

Publication Details

Page(s) 6-10

DOI DOI not available

Published Journal: Pakistan Journal of Science, Volume: 56, Issue: 1--2, Year: 2004

Request the full text from PASTIC