Level of TFEC gene expression in piebald variants of ball python (Python regius)

Thanet Sophonnithiprasert; Ratchanok Kumsiri; Panan Kanchanaphum

doi:doi.org/10.35495/ajab.2025.285

Author(s)

Thanet Sophonnithiprasert Biochemistry Unit, Faculty of Science, Rangsit University,Pathumthani,Thailand

Ratchanok Kumsiri Pathobiology Unit, Faculty of Science, Rangsit University,Pathumthani,Thailand

Panan Kanchanaphum Biochemistry Unit, Faculty of Science, Rangsit University,Pathumthani,Thailand

Abstract

The ball python, or Python regius, is a popular exotic pet known for its beautiful colors and patterns, particularly in the piebald morph. Piebald ball pythons have skin with white patches on a pattern with a brown and black background. The piebald trait results from a recessive mutation in the TFEC gene. There are three variations of piebald: low white, medium white, and high white. This study uses quantitative PCR (qPCR) to determine the levels of TFEC gene expression across these three piebald variants. In the four low white piebald samples analyzed, the reduction of TFEC gene expression ranged from 1.31 to 4.42-fold, compared to the wild type, displaying the lowest reduction fold of TFEC gene expression. This phenomenon shows the pattern on almost all the skin of the ball python. While in medium white, the reduction in TFEC gene expression ranges from 5.14 to 7.99-fold, indicating the equal distribution of patterned and white patches on the skin of the ball python. The last one, the greatest reduction in TFEC gene expression, is the high white piebald ball python. The average reduction of TFEC gene expression is between 17.28 to 48.92-fold, reflecting the almost white area in the skin. Our study is the first report of differential expression of the TFEC gene in the variant type of the piebald ball python.

Publication Details

Page(s) 1-11

DOI doi.org/10.35495/ajab.2025.285

Published Journal: Asian Journal of Agriculture and Biology, Volume: 2, Issue: 0, Year: 2026

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Keywords

TFEC gene, Piebald, qPCR, Ball python, gene expression, Python regius

References

Anello M,Fernández E,Daverio MS,Vidal-Rioja L,Di Rocco F .2019 .TYR Gene in Llamas: Polymorphisms and Expression Study in Different Color Phenotypes. Front. Genet, 12(10) : 568.

Brown AR,Comai K,Mannino D,Mccullough H,Donekal Y,Meyers HC,Graves CW,Seidel HS,Consortium BIO306W .2022 .A community-science approach identifies genetic variants associated with three color morphs in ball pythons (Python regius). PLoS One, 027(10) : 6376.

Garcia-Elfring A,Sabin CE,Iouchmanov AL,Roffey HL,Samudra SP,Alcala AJ,Lauderdale RS,Hendry JD,Menke AP,Barret DB,RDH DB .2023 .Piebaldism and chromatophore development in reptiles are linked to the tfec gene. Curr. Biol, 01(4) : 755-763.

Grossen TL,Bunnam A,Cohen RE .2025 .Circadian Clock Genes in the Lizard Brain. J. Exp. Zool. A Ecol. Integr. Physiol, 343(2) : 278-284.

Groza T,Gomez FL,Mashhadi HH,Muñoz-Fuentes V,Gunes O,Wilson R,Cacheiro P,Frost A,Keskivali-Bond P,Vardal B,McCoy A,Cheng TK,Santos L,Wells S,Smedley D,Mallon AM,Parkinson H .2023 .The International Mouse Phenotyping Consortium: comprehensive knockout phenotyping underpinning the study of human disease. Nucleic Acids Res, 6(D1) : D1038-D1045.

Handi RM,Inamdar DLS .2023 .Sexually dimorphic expression of AMH facilitates testis differentiation pathway in the tropical lizard, Calotes versicolor (Daud. Mol Cell Endocrinol, 577(1) : 112011.

Kanchanaphum P .2018 .Time course of detection of human male DNA from stained blood sample on various surfaces by loop mediated isothermal amplification and polymerase chain reaction. BioMed. Res. Int, 22 : .

Kanchanaphum P .2018 .Identification of human DNA by loop-mediated isothermal amplification (LAMP) technique combined with white ring precipitation of Cu(OH)2. Songklanakarin Journal of Science and Techology, 40 : 738-742.

Kokiattrakool W,Saengcharatuaong N,Luapan J,Sroykham W,Kumsiri R,Kanchanaphum P .2024 .Differentiation between wild type and heterozygous albino ball pythons (Python regius) by PCR and qPCR. Braz. J. Biol, 84 : 286676-6984.

Kumsiri R and Kanchanaphum P .2020 .A Comparison of Four Molecular Methods for Detection of AflatoxinProducing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand. Scientifica (Cairo), 24 : .

Kumsiri R and Kanchanaphum P .2021 .Comparison of time course detection of human male DNA from blood stains on various objects on the surface in a natural environment, and in a laboratory using Loop-Mediated Isothermal Amplification (LAMP). Scientifica (Cairo), 25 : .

Kumsiri R,Yin NN,Kanchanaphum P .2025 .Discrimination between wild type and heterozygous piebald ball pythons (Python regius) by PCR and qPCR. Braz. J. Biol, 85 : 293718-6984.

Kumsiri R,Sophonnithiprasert T,Kanchanaphum P .2025 .Level of TRY gene expression in morph albino in ball python (Python regius). Int. J. Agric Biosci, 14(6) : 1240-1245.

Kuiper RP,Schepens M,Thijssen J,Schoenmakers EF .2004 .Regulation of the MiTF/TFE bHLH-LZ transcription factors through restricted spatial expression and alternative splicing of functional domains. Nucleic Acids Res, 32(8) : 2315-22.

Levy C,Khaked M,Fisher DE .2006 .MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol. Med, 07(9) : 406-414.

Lister JA,Robertson CP,Lepage T,Johnson SL,Raible DW .1999 .nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Development, 126(17) : 3757-67.

Mannan R,Chen YB,Wang X,Zhang Y,Hosseini N,Sangoi AR,Acosta A,Williamson SR,Mahapatra S,Chinnaiyan AK,Hu J,Vaishampayan U,Shao L,Betz BL,Kim AS,Cao X,Su F,Wang R,Argani P,Brown N,Tickoo SK,Chinnaiyan AM,Reuter VE,Dhanasekaran SM,Mehra R .2025 .TRIM63 Overexpression in FISH-Negative MiTF Family Altered Renal Cell Carcinoma (MiTF RCC). Mod Pathol, 38(11) : 100873.

Martina JA,Puertollano R .2016 .TFEB and TFE3: The art of multi-tasking under stress conditions. Transcription, 8(1) : 48-54.

Modahl CM,Han SX,van Thiel J,Vaz C,Dunstan NL,Frietze S,Jackson TNW,Mackessy SP,Kini RM .2024 .Distinct regulatory networks control toxin gene expression in elapid and viperid snakes. BMC Genomics, 16(1) : 186.

Olsson M,Stuart-Fox D,Ballen C .2013 .Genetics and evolution of colour patterns in reptiles. Semin. Cell Dev. Biol, 04(6-7) : 529-541.

Petratou K,Spencer SA,Kelsh RN,Lister JA .2021 .The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor. PLoS One, 02(1) : 44794.

Steingrimsson E,Moore KJ,Lamoreux ML,FerreDerre-D'amare AR ML,Burley SK,Zimring DC,Skow LC,Hodgkinson CA,Arnheiter H,Copeland NG .1994 .Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences. Nat. Genet, 8(3) : 256-263.

Steingrimsson E,Tessarollo L,Pathak B,Hou L,Arnheiter H,Copeland NG,Jenkins NA .2002 .Mitf and Tfe3, two members of the Mitf-Tfe family of bHLH-Zip transcription factors, have important but functionally redundant roles in osteoclast development. Proc Natl Acad Sci USA, 99(7) : 4477-82.

Sun Y,Li Y,Luo D,Liao DJ .2012 .l7 (8): e41659. PLoS ONE, 0041 : 659.

Tassabehji M.,Newton VE,Read AP .1994 .Waardenburg syndrome type 2 caused by mutations in the human microphthalmia (MITF) gene. Nat. Genet, 8(3) : 251-255.

Tzika AC .2024 .On the role of TFEC in reptilian coloration. Front. Cell Dev. Biol, 7(12) : 1358828.

Ullate-Agote A,Tzika AC .2021 .Characterization of the leucistic Texas rat snake Pantherophis obsoletus. Front. Ecol. Evol, 9 : 583236.