DIETARY SELENIUM SUPPLEMENTATION MODULATES PROLIFERATIVE GENE REGULATORS THROUGH ATTENUATING CELL CYCLE INHIBITION ON LATE G1/S TRANSITION IN PRE-PUBERTAL GOAT TESTIS

MUHAMMAD AWAIS SOOMRO; MOOLCHAND MALHI; TARIQUE AHMED KHOKHAR; FAIZ MUHAMMAD KHAND; ZAFAR ALI KHOSO; MARIA SARDAR; MUHAMMAD MOHSEN RAHIMOON; AWAIS BIN SHAHID; MUHAMMAD ANEES MEMON; NABI BUX SOLANGI; JAWED ALI DHILOO

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DIETARY SELENIUM SUPPLEMENTATION MODULATES PROLIFERATIVE GENE REGULATORS THROUGH ATTENUATING CELL CYCLE INHIBITION ON LATE G1/S TRANSITION IN PRE-PUBERTAL GOAT TESTIS

Author(s)

MUHAMMAD AWAIS SOOMRO Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

MOOLCHAND MALHI Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.

TARIQUE AHMED KHOKHAR Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

FAIZ MUHAMMAD KHAND Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

ZAFAR ALI KHOSO Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

MARIA SARDAR Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

MUHAMMAD MOHSEN RAHIMOON Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

AWAIS BIN SHAHID Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.

MUHAMMAD ANEES MEMON Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.

NABI BUX SOLANGI Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.

JAWED ALI DHILOO Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.

Abstract

Selenium (Se) is essential for normal gonadal improvement in prepubertal male goat. Thus hypothesized that dietary Se may influence late G1/S through M-phase gene networks in the testis, thereby advancing pre-pubertal testicular development via attenuation of inhibitory signals and modulation of late G1 cell-cycle regulators. The total of twenty animals, male goats (3 months old) weighing ~11 kg were arbitrarily given to a basal diet (Control; C, n = 10) or the same diet plus Se-yeast (SY; 0.3 mg/kg; n = 10) for 10 weeks. Basal Se concentration in control diet was 0.037 mg/kg (ICP-OES Optima 2100-DV, PerkinElmer). Transcript analysis indicated increases in cyclin E1, Cyclin dependent kinase-2 CDK-2, cyclin B1, cyclin A, and Cyclin dependent kinase-1 CDK-1 by 10.5 %, 12.6 %, 11.6 %, 24 %, and 24 %, respectively (P > 0.05), while the CDK inhibitor p-21 was significantly reduced by 42 % (P < 0.05) in SY vs. C. Because controlled proliferation underpins tissue growth and homeostasis, the marked reduction of p-21 suggests a mechanism through which Se facilitates regulated proliferation without promoting dysplasia. Collectively, organic Se supplementation appears to foster late G1-phase proliferative signaling by relieving cell-cycle inhibition, thereby supporting testicular development in pre-pubertal goats. The data suggest that Se supplementation level modulates proliferative gene expression, thereby attenuating cell cycle inhibition in pre-pubertal testes of male goat.

Publication Details

Page(s) 24-31

DOI DOI not available

Published Journal: Journal of Bioresource Management, Volume: 13, Issue: 1, Year: 2026

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Keywords

Selenium yeast, p-21, CDKs, goat, testis., cell cycle, late G1, cyclins

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