Empagliflozin: HPLC based analytical method development and application to pharmaceutical raw material and dosage form

Anas M. Hanif; Rabia Bushra; Nahlah Elkudssiah Ismail; Rahila Bano; Saima Abedin; Shazia Alam; Maqsood Ahmed Khan; Hafiz Muhammad Arif

doi:10.36721/PJPS.2021.34.3.SUP.1081-1087.1

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Empagliflozin: HPLC based analytical method development and application to pharmaceutical raw material and dosage form

Author(s)

Anas M. Hanif Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University,Karachi,Pakistan

Rabia Bushra Department of Pharmaceutics, Faculty of Pharmacy, Dow University of Health Sciences,Karachi,Pakistan

Nahlah Elkudssiah Ismail Clinical Pharmaceutics Research Unit, Faculty of Pharmacy, MAHSA University,Selangor,Malaysia

Rahila Bano Department of Pharmaceutics, Faculty of Pharmacy, Dow University of Health Sciences,Karachi,Pakistan

Saima Abedin Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University,Karachi,Pakistan

Shazia Alam Department of Pharmaceutics, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan

Maqsood Ahmed Khan Department of Pharmaceutics, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan

Hafiz Muhammad Arif Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan

Abstract

The current investigation is based on efficient method development for the quantification of empagliflozin in raw and pharmaceutical dosage forms, as no pharmacopoeial method for the drug is available so far. The developed analytical method was validated as per ICH guidelines. C18 column with mobile phase (pH 4.8) consisted of 0.1% trifluoroacetic acid solution and acetonitrile (70:30 v/v) was used for drug analysis. The calibration plot showed good linear regression (r2>0.999) over the concentration of 0.025-30 µg mL-1. The LOD and LOQ were found to be 0.020 µg mL-1 and 0.061 µg mL-1, respectively. The percentage recovery was estimated between 98.0 to 100.13%. Accuracy and precision data were found to be less than 2%, indicating the suitability of method for routine analysis in pharmaceutical industries. Moreover, the drug solution was found to be stable in refrigerator and ambient room temperature with mean % accuracy of >98%. Empagliflozin contents were also tested in both the raw API and marketed tablet brands using this newly developed method. The mean assay of raw empagliflozin and tablet brands were ranged from 99.29%±1.12 to 100.95%±1.69 and 97.18%±1.59 to 98.92%±1.00 respectively. Based on these findings, the present investigated approach is suitable for quantification of empagliflozin in raw and pharmaceutical dosage forms.

Publication Details

Page(s) 1081-1087

DOI 10.36721/PJPS.2021.34.3.SUP.1081-1087.1

Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: SUP3, Year: 2021

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Keywords

Validation, Method development, HPLC, Bulk dosage form, Empagliflozin

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