Activity guided isolation and mechanistic approach towards analgesic potential of Chenopodium mediated through opioidergic pathway

Sahar Nasim; Mohib Shah; Syed Wadood Ali Shah; Muhammad Naeem Ahmed; Mubashir Ahmad; Natasha Anwar; Mehreen Ghias

doi:10.36721/PJPS.2021.34.1.SUP.197-203.1

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Activity guided isolation and mechanistic approach towards analgesic potential of Chenopodium mediated through opioidergic pathway

Author(s)

Sahar Nasim Department of Botany, Abdul Wali Khan University Mardan,Khyber Pakhtunkhwa,Pakistan;Department of Botany, University of Malakand,Khyber Pakhtunkhwa,Pakistan

Mohib Shah Department of Botany, Abdul Wali Khan University Mardan,Khyber Pakhtunkhwa,Pakistan

Syed Wadood Ali Shah Department of Pharmacy, University of Malakand,Khyber Pakhtunkhwa,Pakistan

Muhammad Naeem Ahmed Department of Chemistry, University of Azad Jammu and Kashmir,Muzaffarabad,Pakistan

Mubashir Ahmad Department of Pharmacy, University of Malakand,Khyber Pakhtunkhwa,Pakistan

Natasha Anwar Department of Chemistry, Abdul Wali Khan University Mardan,Khyber Pakhtunkhwa,Pakistan

Mehreen Ghias Department of Pharmacy, University of Malakand,Khyber Pakhtunkhwa,Pakistan

Abstract

The current study is focused towards screening for its phytochemicals, phenolic and flavonoid contents of different species of Chenopodium. The plants were also screened for corroborating the traditional use of medicinal plants locally used for pain by determining the extract and their fractions for the in-vivo analgesic activity by using the modern scientific system. Among chloroform fractions, a high level of total phenolic contents was found in chloroform fraction of Chenopodium ambrosioides (ChAm-Chf) with 57.12±1.02 followed by Chenopodium botrys (ChBt-Chf) with 56.79±0.71. High content of flavonoids was found in chloroform fraction of Chenopodium botrys (ChBt-Chf) extract with 78.35±0.84 followed by Chenopodium ambrosioides (ChAm-Chf) with 75.20±0.81. The crude extract Chenopodium album, Chenopodium botrys and Chenopodium ambrosioides (ChAl-Crd, ChBt-Crd and ChAm-Crd) at 100 and 200 mg/kg, chloroform and ethylacetate fractions (ChAl-Chf, ChBt-Chf, ChAm-Chf, ChAl-Et, ChBt-Et and ChAm-Et) at 75 mg/kg caused significant inhibition (P<0.05, P<0.01, P<0.001, n=8) of the analgesic response induced by acetic acid, formalin and hotplate method. Mechanistically, the naloxone overturns completely the analgesic effects of beta-sitosterol (SN2) while partial reversal was observed by ursolic acid (SN1) indicating other possible mechanisms in association with opioid receptors.

Publication Details

Page(s) 197-203

DOI 10.36721/PJPS.2021.34.1.SUP.197-203.1

Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: SUP1, Year: 2021

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Keywords

in-vivo animal model, Chenopodium, Pain, phenolics and flavonoids

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