Multivariate analysis, morphological characterization and in vitro organogenesis in elite varieties of carnation (dianthus caryophyllus)

SEHRISH SOMAYA; MONIS HUSSAIN SHAH; RIAZ-UR-REHMAN; ZIA ULLAH; SAMIA IKRAM

doi:10.30848/PJB2025-6(25

Author(s)

SEHRISH SOMAYA Horticultural Research Institute for Floriculture and Landscaping,Rawalpindi, Islamabad, 45510,Pakistan

MONIS HUSSAIN SHAH Horticultural Research Institute for Floriculture and Landscaping,Rawalpindi, Islamabad, 45510,Pakistan

RIAZ-UR-REHMAN Directorate of Floriculture, (T & R),Punjab, Lahore, 45000,Pakistan

ZIA ULLAH Horticultural Research Institute for Floriculture and Landscaping,Rawalpindi, Islamabad, 45510,Pakistan

SAMIA IKRAM Horticultural Research Institute for Floriculture and Landscaping,Rawalpindi, Islamabad, 45510,Pakistan

Abstract

Plant tissue culture techniques can be successfully adopted for rapid multiplication of the exclusive indigenous planting material. This study was conducted at the Plant Tissue Culture laboratory of Horticultural Research Institute for Floriculture and Landscaping, Islamabad, during the year 2018-2021. Three carnation varieties named Antigua, Montoyo and Tabasco were used as an explant material. Different combinations of growth regulators 6-Benzylaminopurine (BAP) and Naphthaleneacetic acid (NAA) were used to investigate the optimum concentration of both hormones for best shoot proliferation protocol. Each variety behaved differently, producing variable results against the parameters tested. In case of Antigua, the combination of 4.0 mg/L BAP + 0.5 mg/L NAA proved to be the best by producing maximum number of shoots, shoot length and shoot proliferation percentage whereas, Montoyo and Tabasco showed satisfactory results on the medium supplemented with 3.0 mg/L BAP + 1.5 mg/L NAA and 4.0 mg/L BAP + 1.5 mg/L NAA respectively. After successful standardization of shoot proliferation protocol, the plantlets were transferred to the ½ strength MS medium enriched with 0.25 mg/l Indole-3-Butyric Acid (IBA) for root initiation. The rooted plantlets were transplanted in the small cells containing mixture of soil and leaf manure (1:1) for acclimatization.

Publication Details

Page(s) 2243-2249

DOI 10.30848/PJB2025-6(25

Published Journal: Pakistan Journal of Botany, Volume: 57, Issue: 6, Year: 2025

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Keywords

Trade, Novel, Commercial, flowers, In vitro propagation

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