Background: Bone Marrow Transplantation (BMT) is a curative treatment for numerous malignant and nonmalignant hematologic disorders. Nurses are pivotal in patient care, requiring up-to-date knowledge and practical competency in BMT-related management. Objective: To assess the effect of an educational program on the knowledge and practice of nurses caring for patients undergoing BMT. Methodology: A quasi-experimental design involving a convenience sample of 34 nurses from the BMT Unit at Hiwa Cancer Hospital, from March 2023 to September 2024 was adopted. Data were collected using a structured questionnaire comprising demographic details, knowledge assessment and a practice evaluation. Participants attended an 8-session educational program designed to enhance their competencies. Results: Baseline assessments revealed that most participants had poor knowledge (67.6%) and practice (73.5%), with mean scores of 0.42±0.23 and 0.63±0.42, respectively. Post-program evaluations showed a substantial improvement in knowledge, with 73.5% of nurses classified as having good knowledge (mean score of 0.79±0.12) (p<0.001). Similarly, nurses demonstrating good practices increased from 5.9% to 41.2%, significantly increasing the mean practice score to 1.40±0.29 (p<0.001). Conclusions: The educational program effectively enhanced the knowledge and practice of nurses managing patients undergoing BMT at Hiwa Cancer Hospital.