SLC19A1 Gene Polymorphism; Risk Factor for Preeclampsia

Iram Nasir; Amena Rahim; Muhammad Afzal; Sadaf Ayub; Zahira Bashir; Naila Zakria

doi:10.29271/jcpsp.2025.03.306

Author(s)

Iram Nasir Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Amena Rahim Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Muhammad Afzal Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Sadaf Ayub Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Zahira Bashir Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Naila Zakria Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan

Abstract

Objective: To determine the genotypic and allelic frequency of SLC19A1 (rs1051296 G>T) polymorphism in pre-eclamptic patients and normal pregnant females, and also the genetic association of SLC19A1 (rs1051296 G>T) polymorphisms with the levels of homocysteine (Hcy) and folate in pre-eclamptic Pakistani population. Study Design: A case-control study. Place and Duration of the Study: Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan, from September 2022 to 2023. Methodology: Gene association of SLC19A1 (rs1051296 G>T) polymorphism was studied in pre-eclamptic patients to elucidate its clinical signi cance. The study included 166 pre-eclamptic patients and 166 normotensive pregnant females as controls. Maternal venous blood samples were obtained between 20th and 40th weeks of gestation and DNA was extracted using the Chelex method. Allele-speci c polymerase chain reaction (PCR) was used to analyse theSLC19A1 (rs1051296 G>T) polymorphism. Data were analysed using the Chi-square and Student s t-test. Results: A signi cant association in terms of susceptibility was found betweenSLC19A1 (rs1051296 G>T), CA (p <0.03), and AA (p <0.001) genotype with preeclampsia (PE). A signi cant association in terms of protection was found betwSeLeCn19Ath1e (rs1051296 G>T) CC genotype and PE. Notably, low folic acid along with CA (p <0.05) genotype is a high-risk factor for PE. A prior family history of high blood pressure had not proved any signi cant association with SLC19A1 (rs1051296 G>T, p <0.6). Conclusion: SLC19A1 (rs1051296 G>T) polymorphism is a risk factor for PE in the Pakistani population.

Publication Details

Page(s) 306-312

DOI 10.29271/jcpsp.2025.03.306

Published Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 35, Issue: 3, Year: 2025

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Keywords

Blood pressure, single nucleotide polymorphism, Preeclampsia, Polymerase chain reaction, SLC19A1

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