DNA: marker for Leaf Rust disease in wheat.

Tanwir Ahmad Malik; Azhar Iqbal; Muhammad Aslam Chowdhry; Muhammad Kashif; Sajid-Ur-Rahman

No Detail Found!

DNA: marker for Leaf Rust disease in wheat.

Author(s)

Tanwir Ahmad Malik Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Azhar Iqbal Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Muhammad Aslam Chowdhry Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Muhammad Kashif Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Sajid-Ur-Rahman Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Abstract

Leaf (Brown) rust is major disease of wheat in Pakistan as well as in other countries. The objective of the study was to find DNA marker(s) linked to leaf rust resistance trait for use in tailoring disease resistant wheat cultivar. PCR technique and bulked segregant analysis was used to identify DNA marker linked to leaf rust resistant gene in F2 segregating `population. The F2 population derived from the cross involving leaf rust susceptible, SK-7 and resistant parent, PBG-8981 with Lr29 resistant gene was scored for disease resistance. The F2 population segregated into resistant and susceptible plants in a ratio of 3:1 showing monogenic inheritance. The primer 60-5 amplified a polymorphic molecules of 1100 base pairs from the genomic DNA of resistant plant. his DNA molecule can be used as DNA marker to, identify leaf rust resistant plants in a breeding programme for developing rust resistant wheat cultivars.

Publication Details

Page(s) 239-243

DOI DOI not available

Published Journal: Pakistan Journal of Botany, Volume: 39, Issue: 1, Year: 2007

Request the full text from PASTIC