Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Medium Supplementation Improves In Vitro Maturation and Subsequent Embryonic Development of Bufalo Oocytes

Jun Zhang; Meizhen Ou; Yingqi Li; Junming Sun; Yiqiang Ouyang

doi:10.17582/journal.pjz/20241208092220

Author(s)

Jun Zhang Laboratory Animal Center, Guangxi Medical University,Nanning, 530021,China

Meizhen Ou Laboratory Animal Center, Guangxi Medical University,Nanning, 530021,China

Yingqi Li Laboratory Animal Center, Guangxi Medical University,Nanning, 530021,China

Junming Sun Laboratory Animal Center, Guangxi Medical University,Nanning, 530021,China

Yiqiang Ouyang Laboratory Animal Center, Guangxi Medical University,Nanning, 530021,China

Abstract

Previous studies have shown that conditioned medium of bone marrow-derived mesenchymal stem cells (BMSCs) improved in vitro maturation (IVM) of mouse oocytes. However, the efects of BMSCconditioned medium (BMCM) on IVM and subsequent embryonic development of bufalo oocytes remain unclear. In this study, we examined the impacts of BMCM on maturation eficiency and embryonic development of bufalo oocytes after parthenogenetic activation. The results showed that BMCM that was collected on day 3 and added to IVM medium at a 10% addition concentration enhanced IVM and subsequent parthenogenetic development of bufalo oocytes. The present study serves a basis of obtaining high quality recipient oocytes for bufalo somatic cell nuclear transfer .

Publication Details

Page(s) 871-878

DOI 10.17582/journal.pjz/20241208092220

Published Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 2, Year: 2025

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Keywords

In vitro maturation, Bufalo, Oocytes, Bone marrow-derived Mesenchymal stem cells, Conditioned medium

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