Exploring gene polymorphisms associated with otitis media through variable number tandem repeats (VNTR) region

Safa Amer Khalil; Kawther M. A. Hasan

Author(s)

Safa Amer Khalil College of Science for Women, University of Babylon , Iraq

Kawther M. A. Hasan College of Science for Women, University of Babylon , Iraq

Abstract

Background: Otitis is an inflammation and infection of the inner lining of the middle ear. The problem known as suppurative otitis media if there are droplets and holes in the tympanic membrane due to this inflammation and infection of the mucous membrane. The present study focused on genes potentially associated with otitis media in 60 subjects clinically diagnosed with OM The study focuses on two genes, MUC5B and IL-1RN, mutations assessed through variable number tandem repeat (VNTR) regions. Methods: Variable numbers of tandem repeats (VNTR) regions were used to genotype 60 patients with otitis media and 30 healthy individuals for genotypic polymorphisms for the MUC5B and IL-1RN genes through variable number tandem repeat (VNTR) regions Blood samples were collected from participants, and three milliliters were placed directly into EDTA tubes. Subsequently, PCR products were detected by agarose gel electrophoresis and visualized by ethidium bromide staining. The size of amplified DNA fragments was determined by comparison with a 100-bp DNA ladder molecule size marker. Results: This study presents the association of gene polymorphisms with otitis media by genotyping a total of 60 patients as OM, while 30 individuals were noted to be healthy. This was to determine genotypic polymorphisms for the two genes, that is, MUC5B and IL1RN, within the VNTR region. The results showed that the carriers of allele 2 in the genotypic position had nearly a twofold increased risk of developing otitis media. Conclusion: Polymorphism of the MUC5B and IL-1RN genes can be responsible for the severity of the inflammatory process in the middle ear, complicated by otitis media or hearing loss

Publication Details

Page(s) 613-618

DOI DOI not available

Published Journal: Advancements in life sciences, Volume: 11, Issue: 3, Year: 2024

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Keywords

otitis media, Gene polymorphism, MUC5B, IL- 1RN, VNTR

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