Iron-based nanoparticles synthesis, characterization, and antimicrobial effectiveness

Hisham Faiz Jaha; Yasir Anwar; Saleh M. Al-Maaqar; Tahseen Kamal; Sher Bahadar Khan

Author(s)

Hisham Faiz Jaha Department of Biological Sciences, Faculty of Science, King Abdulaziz University Jeddah , Saudi Arabia

Yasir Anwar Department of Biological Sciences, Faculty of Science, King Abdulaziz University Jeddah Saudi Arabia; Center of Excellence in Bionanoscience Research, King Abdul Aziz University, Jeddah Saudi Arabia

Saleh M. Al-Maaqar Department of Biological Sciences, Faculty of Science, King Abdulaziz University Jeddah , Saudi Arabia;Faculty of Education,Department of Biology, AlbaydhaUniversity, Al-Baydha , Yemen

Tahseen Kamal King Abdulaziz University, P. O. Box 80203 Jeddah 21589 - Saudi Arabia

Sher Bahadar Khan Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah , Saudi Arabia

Abstract

Background Antimicrobial resistance (AMR) has emerged as a significant and pressing public health concern, posing serious challenges to effectively preventing and treating persistent diseases. Developing new antibiotics with different mechanisms of action is crucial to effectively address challenges in treating infections. A lot of work has already been done on mono-metallic nanoparticles to address the issue. Methods: This study aimed to synthesize multi-metallic iron, silver, and chitosan-embedded nanoparticles using a green approach. Iron, silver, chitosan nanoparticles, and a composite of iron-silver-chitosan was also synthesized. The synthesized nanoparticles and composites were characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and Fourier Transform Infrared Spectroscopy (FTIR) to evaluate their structural parameters. Their antimicrobial efficacy was investigated through MIC (minimum inhibitory concentration), MBC (minimum bactericidal concentration), and well-disk diffusion assays against Pseudomonas aeruginosa, Acinetobacter baumannii , Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. Results: The size of the Cu-NPs, Cu-Ag NPs, and Cu-Ag-CS NPs were found to be in the range of 32-40 nm size with a spherical shape. The nanocomposites' MIC and MBC were calculated to be 125 µg/mL and 500 µg/mL, respectively. The nanocomposites exhibited a range of clear inhibition zones, with a minimum diameter of 12 ± 0.5 mm and a maximum diameter of 22 ± 0.5 mm. Conclusion: The iron-silver-chitosan nanocomposite has been shown to have significant antimicrobial effects in the laboratory environment compared to other nanoparticles hence can be applied as potential biomedical/biological candidates in future.

Publication Details

Page(s) 525-532

DOI DOI not available

Published Journal: Advancements in life sciences, Volume: 11, Issue: 2, Year: 2024

Request the full text from PASTIC

Keywords

antibacterial, Nanoparticles, Iron, Antifungal agents, Silver Chitosan

References

Dadgostar P .2019 .Antimicrobial resistance: implications and costs. Infection and drug resistance, : 3903-3910.

European .2019 .Center for Disease Prevention and Control. Surveillance of antimicrobial resistance in Europe 2018. , : .

Prestinaci F,Pezzotti P,Pantosti A .2015 .Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and global health, 109(7) : 309-318.

Control CfD .2014 .Improving Antibiotic Use Among Hospitalized Patients. MMWR March, : 763.

Chokshi A,Sifri Z,Cennimo D,Horng H .2019 .Global contributors to antibiotic resistance. Journal of global infectious diseases, 11(1) : 36.

Ahmad A,Viljoen A,Chenia H. .2015 .The impact of plant volatiles on bacterial quorum sensing. Letters in applied microbiology, 60(1) : 8-19.

Al-Masaudi SB,Al-Maaqar SMS .2020 .Susceptibility of multidrug-resistant enteric pathogenic diarrheal Bacteria to Saudi Honey. Journal of King, 32(1) : 47-64.

Al-Maaqar SM,Al-Johny BO,Al-Sharif FD,Al-Shaeri M,ALKenani N M .2022 .Sensitivity of multidrug-resistant pathogenic bacteria to ethanolic extract of Ziziphus spinachristi L. (Sidr) leaves. Bioscience Research, 19(3) : 1467-1475.

Kirui DK,Weber G,Talackine J,Millenbaugh NJ .2019 .Targeted laser therapy synergistically enhances efficacy of antibiotics against multi-drug resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Nanomedicine: Nanotechnology, Biology and Medicine, : 20102018.

Yousefi B,Eslami M,Ghasemian A,Kokhaei P,Sadeghnejhad A .2019 .Probiotics can really cure an autoimmune disease? Gene Reports, (. , : 15100364.

Khan S,Alam F,Azam A,Khan AU .2012 .Gold nanoparticles enhance methylene blue-induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm. International journal of nanomedicine, : 3245-3257.

Ashrafi M,Bayat M,Mortazavi SP,Hashemi SJ,Meimandipour A .2022 .Antimicrobial effect of chitosan silver copper nanocomposite on Candida albicans in immunosuppressive rats. Veterinary Clinical Pathology The Quarterly Scientific Journal, 16(61) : 15-27.

Ashrafi M,Bayat M,Mortazavi P,Hashemi SJ,Meimandipour A .2020 .Antimicrobial effect of chitosan-silvercopper nanocomposite on Candida albicans. Journal of Nanostructure in Chemistry, : 1087-95.

Mohammed MA,Syeda JT,Wasan KM,Wasan EK .2017 .An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics, 9(4) : 53.

Eslami M,Vahabi V,Peyghan AA .2016 .Low-dimensional Systems. , : 766-11.

Eslami M,Peyghan AA .2015 .DNA nucleobase interaction with graphene like BC3 nano-sheet based on density functional theory calculations. Thin Solid Films, : 58952-56.

Palza H .2015 .Antimicrobial polymers with metal nanoparticles. International journal of molecular sciences,, 16(1) : 2099-2116.

Ismail RA,Sulaiman GM,Abdulrahman SA,Marzoog TR .2015 .Antibacterial activity of magnetic iron oxide nanoparticles synthesized by laser ablation in liquid. , : 53286-297.

Pal S .2014 .Antimicrobial activity of iron oxide nanoparticles. , : .

Mohan P,Mala R .2019 .Comparative antibacterial activity of magnetic iron oxide nanoparticles synthesized by biological and chemical methods against poultry feed pathogens. Materials Research Express, 6(11) : 115077.

Burdușel ,Gherasim O,Grumezescu AM,Mogoantă L,Ficai A .2018 .Biomedical applications of silver nanoparticles: an up-to-date overview. Nanomaterials, 8(9) : 681.

Youssef A,Mohamed S,Abdel-Aziz M,Abdel-Aziz M,Turky G .2016 .Biological studies and electrical conductivity of paper sheet based on PANI/PS/Ag-NPs nanocomposite. , : 147333-343.

Kulatunga D,Dananjaya S,Godahewa G,Lee J,De Zoysa M. J .2017 .Chitosan silver nanocomposite (CAgNC) as an antifungal agent against Candida albicans. Medical Mycology, 55(2) : 213-222.

Rhim ,Hong S-I,Park H-M .2006 .Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity. Journal of agricultural and food chemistry, 54(16) : 5814-5822.

Shahbazi Y,Shavisi N. .2019 .Current advancements in applications of chitosan based nano-metal oxides as food preservative materials. Nanomedicine Research Journal, 4(3) : 122-129.

Mirhashemi AH,Bahador A,Kassaee MZ,Daryakenari G,Ahmad-Akhoundi MS .2013 .Antimicrobial effect of nanozinc oxide and nano-chitosan particles in dental composite used in orthodontics. Journal of Medical Bacteriology, 2(3) : 1-10.

Vasylkiv O,Bezdorozhev O,Sakka Y. .2016 .Synthesis of iron oxide nanoparticles with different morphologies by precipitation method with and without chitosan addition. Journal of the Ceramic Society of Japan, 124(4) : 489-494.

Freire PL,Albuquerque AJ,Farias IA,da Silva TG,Aguiar JS .2016 .Antimicrobial and cytotoxicity evaluation of colloidal chitosan-silver nanoparticles-fluoride nanocomposites. International journal of biological macromolecules, : 93896-903.

Soares PI,Machado D,Laia C,Pereira LC,Coutinho JT .2016 .Thermal and magnetic properties of chitosan-iron oxide nanoparticles. Carbohydrate polymers, : 149382-390.

Elshikh M,Ahmed S,Funston S,Dunlop P,McGaw M .2016 .Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology letters, 38(6) : 1015-1019.

Elshikh M,Ahmed S,Funston S,Dunlop P,McGaw M .2016 .Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology letters, : 381015-1019.

Mustapha S,Ndamitso M,Abdulkareem A,Tijani J,Shuaib D .2019 .Comparative study of crystallite size using Williamson-Hall and Debye-Scherrer plots for ZnO nanoparticles. Advances in Natural Sciences: Nanoscience and Nanotechnology, 10(4) : 045013.

Gomathi M,Rajkumar P,Prakasam A .2018 .Study of dislocation density (defects such as Ag vacancies and interstitials) of silver nanoparticles, green-synthesized using Barleria cristata leaf extract and the impact of defects on the antibacterial activity. Results in Physics, : 10858-864.

Unsoy G,Yalcin S,Khodadust R,Gunduz G,Gunduz U .2012 .Synthesis optimization and characterization of chitosancoated iron oxide nanoparticles produced for biomedical applications. Journal of Nanoparticle Research, : 141-13.

Kalarical Janardhanan S,Ramasamy I,Nair BU .2008 .Synthesis of iron oxide nanoparticles using chitosan and starch templates. Transition Metal Chemistry, : 33127-131.

Sarkar S,Das R .2019 .Synthesis of silver nano-cubes and study of their elastic properties using X-ray diffraction line broadening. Journal of Nondestructive Evaluation, : 381-8.

Nikpour M,Rabiee S,Jahanshahi M. Synthesis .2012 .and characterization of hydroxyapatite/chitosan nanocomposite materials for medical engineering applications. , 43(4) : 1881-1886.

Jayalekshmi A,Victor SP,Sharma CP .2013 .Magnetic and degradable polymer/bioactive glass composite nanoparticles for biomedical applications. , : 101196-204.

Rui D,Rui R,Liu YL,Ying Z,Guo LN .2023 .Single-Center Retrospective Study. Biomedical and Environmental Sciences, 36(8) : 732-742.

Mariani F,Galvan EM .2023 .Staphylococcus aureus in Polymicrobial Skinand Soft Tissue Infections: Impact of Inter-Species Interactionsin Disease Outcome. , 12(7) : 1164.

Talapko J,Juzbašić M,Matijević T,Pustijanac E,Bekić S .2021 .Candida albicans-the virulence factors and clinical manifestations of infection. Journal of Fungi, 7(2) : 79.

Seddighi NS,Salari S,Izadi AR .2017 .Evaluation of antifungal effect of iron‐oxide nanoparticles against different Candida species. Iet Nanobiotechnology, 11(7) : 883-888.

Rudramurthy GR,Swamy MK,Sinniah UR,Ghasemzadeh A .2016 .Nanoparticles: alternatives against drug-resistant pathogenic microbes. Molecules, 21(7) : 836.

Ahmad A,Wei Y,Syed F,Tahir K,Rehman AU .2017 .The effects of bacteria-nanoparticles interface on the antibacterial activity of green synthesized silver nanoparticles. Microbial pathogenesis, : 102133-142.

Arias LS,Pessan JP,Vieira APM,Lima TMTd,Delbem ACB .2018 .Iron oxide nanoparticles for biomedical applications: A perspective on synthesis, drugs, antimicrobial activity, and toxicity. , 7(2) : 46.

Lavaee F,Faez K,Faez K,Hadi N,Modaresi F .2016 .Antimicrobial and antibiofilm activity of silver, titanium dioxide and iron nano particles. Am J Dent, 29(6) : 315-320.