Hematological effects for rabbits immunized by Corynebacterium pseudotuberculosis sonicated antigen

Basil R. F. Razook; Mariam Hamdi Abdulkareem; Ansam Khalid Mohammed

Author(s)

Basil R. F. Razook College of Veterinary Medicine,University of Baghdad , Iraq

Mariam Hamdi Abdulkareem Department of Microbiology, College of Veterinary Medicine,University of Baghdad , Iraq

Ansam Khalid Mohammed Department of Microbiology,College of Veterinary Medicine,University of Baghdad , Iraq

Abstract

Background: Analyzing Hematological parameters is usually in use to monitor various circumstances such as infection, inflammation and anemia. For that we studied blood parameters which are in touch with immunized laboratory animals (rabbits) after immunizing these animals with Corynebacterium pseudotuberculosis then used an adjuvant of Pseudomonas aeruginosa to stimulate the immune response cells after 2 to 3 weeks of immunization. Different concentrations were used to examine the effect on the animals’ blood parameters changing. Methods: Corynebacterium pseudotuberculosis bacterium was used at current study for evaluating the effect of immunizing laboratory rabbits with two different immune stimulators working together as adjuvant on the blood picture with the aid of 12 breed rabbits from different genders, dividing them at the base of inoculation with pre-killed and sonicated cells of bacteria (antigens) onto 4 categories as following: the group-1 members of rabbits were injected with the antigen, group-2 and group-3 included rabbits injected with the two inoculations at various concentrations; final group, group-4 was the control group. Results: Thus, white blood cells known to be our main line of defenses act firstly in our body towards different infecting microorganisms, their count always was increased through the immunization correlated with other measured parameters. From the results we can see that the lymphocytes percentage had no alteration between the infected and the immunized compared to control group, while the percentage of MID that refers to the monocytes was at the same range in both infected and the immunized control rabbit’s groups. Granulocyte cells percentage, which is including heterophilic, basophilic and eosinophilic cells, was significantly decreased (P>0.05) compared with control at all groups. nclusion: We conclude that C. pseudotuberculosis might function as a potent immunogen to notice the complete blood picture variations. .

Publication Details

Page(s) 375-379

DOI DOI not available

Published Journal: Advancements in life sciences, Volume: 11, Issue: 2, Year: 2024

Request the full text from PASTIC

Keywords

Blood parameters, Corynebacterium pseudotuberculosis Pseudomonas aeruginosa Sonicated antigen, Blood picture Adjuvant

References

Baird GJ,Fontaine MC .2007 .Corynebacterium pseudotuberculosis and its role in ovine caseous lymphadenitis. Journal of Comparative Pathology, 37 : 179-210.

HR Carne .2007 .The toxin of Corynebacterium bovis. Journal of Pathology and Bacteriology, 51 : 199-212.

.1988 .Biochemical andgenetic characterization of Corynebacterium pseudotuberculosis. American Journal of Veterinary Research, 49 : 221-6.

GC Hard .1975 .Comparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages. Infection and Immunity, 12 : 1439-49.

Paton MW .2010 .The Epidemiology and Control of Caseous Lymphadenitis inAustralian Sheep Flocks. PhD thesis, : .

Titball RW,Microbiology C. .2012 .. , 57 : 13-66.

Abdullah FFJ,Latif NAA,Chung ELT,Aimi S,Sarah MZS,Haron AW,Lila MAM,Zakaria Z,Norsidin MJ Z .2015 .Changes in the reproductive hormones of non-pregnant does infected intradermally with Corynebacterium pseudotuberculosis in chronic form. International Journal of Livestock Research, 5(7) : 33-40.

Othman AM,Abdullah FFJ,Nordi M,Rina NA,Ilyasu Y,Zamri-Saad M,Wahid AH,Saharee AA,Mohd-Azmi ML .2014 .Biochemical and serum electrolyte changes in nonpregnant boer does inoculated with Corynebacterium pseudotuberculosis via various routes. IOSR Journal of Agriculture and Veterinary Science, 7(10) : 5-8.

Zeru F,Kahsay AG .2014 .Caseous lymphadenitis in goats from Borena range Land South Ethiopia slaughtered at luna export abattoir. Journal of. Veterinary Medicine and Animal Health, 6(6) : 168-173.

Abdullah FFJ,Osman AY,Adamu L,Zakaria Z,Abdullah R,Saad MZ,Saharee AA .2013 .Acute phase protein profiles in calves following infection with whole cell, lipopolysaccharides, and outer membrane protein extracted from Pasteurella multocida Type B:2. Asian Journal of Animal and Veterinary Advances, 8(4) : 655-662.

Junior JP,Oliveira A,Alves F,Silva L,Rabelo S,Mota R. .2006 .Corynebacterium pseudotuberculosis experimental infection of goats mamary gland. Arquivos do Instituto Biológico, 73(4) : 395-400.

Latif NAA,Abdullah FFJ,Othman AM,Rina A,Chung ELT.,Zamri-Saad M,Saharee AA,Haron AW,Lila MAM AW .2015 .Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form. , 8(7) : 924.

Collins MD,Burton RA,Jones D. .1988 .A new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiology Letters, 49(3) : 349-352.

Marrakchi H,Lanéelle MA,Daffé M. .2014 .Mycolic acids: Structures, biosynthesis, and beyond. Chemical Biology, 21(1) : 67-85.

Vasco A .2012 .Corynebacterium pseudotuberculosis: Immunological responses in animal models and zoonotic potential. Journal of Clinical and Cellular Immunology, 1(S4) : 5.

Abdulkareem MH,Razook BRF.,AL-Rubaiey MGA .2022 .Histopathological Changes in few Digestive Organs Affected by Corynebacterium pseudotuberculosis. Biochemical and Cellular Archives, 22(2) : 3743-3750.

Ozkanlar Y,Aktas M,Kaynar O,Ozkanlar S,Kireccl E. .2012 .Bovine respiratory disease in naturally infected calves: Clinical signs, blood gases and cytokine response. , 163 : 123-130.

Pazatsen R,Shun S,Abdullah FFJ,Saad MZ,March MZ .2012 .Haron A. Clinical Response and Pathological Changes Associated with Corynebacterium pseudotuberculosis Infection in Mice. UPM Serdang, Malaysia. 7th Proceedings of the Seminar in Veterinary Sciences, 27 : February.

Motiva I,Denchen V .1992 .Linda K. Humeral and mediated immunity in mice after immunization with live oral vaccine of Salmonella typhimurium: auxotrophic mutant with two attenuating markers. Vaccine.Microbiology, : 61-66.

Jayapal V,Medical Publishers LTD V .2007 .Fundamentals of Medical Immunology. Jaypea Brothers, : 180-189.

Scavone P,Miyoshi PA,Rail a,Chabalgoity A,langella P,Azerdo V,Zunino P. .2007 .Intranasal immunization with recombinant Lactococcus lactis displaying either anchored or secreted forms of Proteus mirabilis - Mrp A fimbriae protein confers specific immune response and induces a significant reduction of kidney bacterial colonization in mice. Microbes and Infection, 9 : 821-828.

W.B.Saunders Company. .1974 .. , : 70-83.

Al-Samarrae EA .2011 .Evaluation of Proteus vulgaris fimbriae antigen by delayed type hypersensitivity (DTH)-skin test in rabbits. Iraqi Journal of Veterinary Medicine, 35(1) : 100-106.

Rzook BRF,Al-Samarrae IAA,Al-Rubaie HMA .2015 .The Effect of Proteus vulgaris Sonicate fimbriae antigens in some blood parameters and humoral immune response. Baghdad Science Journal, 12(2) : 301-306.

Veterinary World .2015 .. , 8(9) : 1105.

Mahmood ZK,Jin ZAM,Jesse FF,Saharee AA,Sabri J,Yusoff R,Haron AW .2016 .Relationship between the Corynebacterium pseudotuberculosis phospholipase D inoculation and the fertility characteristics of crossbred Boer bucks. Livestock Science, 191 : 12-21.

Abdulkareem MH,Razook BRF,Abdul Ameer AH,Alrubaye B. Histopathological .2022 .Changes of Rabbits' Vital Organs Associated with Corynebacterium Pseudotuberculosis Infection. International Journal of Special Education, 37(3) : 13243-13255.

Abdulkareem MH,Razook BRF,Taher DD .2022 .; 49 special issue. Indian Journal of Ecology, 19 : 230-233.

Experimental Clinical Sciences Journal,Turgut A,Sak ME,Ozler A .2013 .Alterations of peripheral blood cells in tubal ectopic pregnancy. Ginekologia Polska, 84 : 193-196.

Gasparyen AY,Ayvazyen L,Mikhalidis D. .2011 .MPV: A link between thrombosis and inflammation. Current Pharmaceutical Design, 17(1) : 47-58.

Pseudotuberculosis .2012 .. Advances in Animal and Veterinary Sciences, 11(24) : 4660-4667.

Russell KE,Grindem CB .2000 .Secondary thrombocytopenia. In: Schalm's Veterinary Hematology. Lippincott Williams and Wilkins, : 487-495.

. .To read the copy of this license please visit: https://creativecommons. , : .