Indole-3-Carbinol of Broccoli Powder Can Decrease Estrogen Production in Male Rats

Reski Amalia; Claude Mona Airin; Pudji Astuti; Agung Budiyanto

doi:10.17582/journal.jahp/2024/12.4.647.653

Author(s)

Reski Amalia Doctoral Student, Veterinary Science Postgraduate Programme, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Karangmalang, Sleman, Yogyakarta 55281 Indonesia

Claude Mona Airin Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Karangmalang, Sleman, Yogyakarta 55281, Indonesia

Pudji Astuti Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Karangmalang, Sleman, Yogyakarta 55281, Indonesia

Agung Budiyanto Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Karangmalang, Sleman, Yogyakarta 55281, Indonesia.

Abstract

The increasing use of plants as a form of medicine presents fewer risks today. Broccoli (Brassica oleracea var. italica) has been shown to influence various physiological processes, including hormonal modulation. This study aimed to evaluate the efect of broccoli powder on estradiol (E2) levels in male rats. Over a period of 28 days, male rats were orally administered broccoli powder containing indole-3-carbinol (I3C). A total of 25 rats were divided into 5 groups (P1 to P5) and supplemented with either distilled water (negative control), letrozole (positive control), or broccoli powder at dosages of 0.117 g/kg BW, 0.234 g/kg BW, and 0.460 g/kg BW. Weekly blood samples and body weight measurements were taken over the 4-week period, and necropsy was performed in the final week to collect the testicles and brain. Testis and body weight were quantified, and E2 levels were measured by ELISA. Results showed that body weight was significantly higher (p<0.05) in groups P1 (192.32 ± 2.85g), P2 (199.96 ± 3.02g), and P3 (206.56 ± 2.96g) compared to groups P1 (179.29 ± 2.91g) and P2 (185.48 ± 2.75g). However, no significant diferences (p>0.05) were observed in brain weight, testis weight, or testis length. The broccoli powder significantly afected E2 levels in male rats, with notable (p<0.05) efects in groups P4 and P5 during Week 4, showing values of 2.99 ± 0.49 pg/ml and 2.38 ± 0.48 pg/ml, respectively. These findings suggest that broccoli powder administration impacts body weight and deccreases E2 concentration in male rats, potentially benefiting reproductive success and function.

Publication Details

Page(s) 647-653

DOI 10.17582/journal.jahp/2024/12.4.647.653

Published Journal: Journal of animal health and production, Volume: 12, Issue: 4, Year: 2024

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Keywords

testosterone, decrease, estradiol, Broccoli, Indole-3-carbinol, CYP19, | Aromatase blocker

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