This study deals with the sequence analysis, high-level expression, single step purification and refolding of growth hormone of a local caprine breed “Beetal”. Sequence analysis of caprine growth hormone (cGH) gene showed several nucleotide and two amino acid variations in cDNA of cGH with the previously reported caprine GH sequence. cGH was found to be more closely related phylogenetically to sheep, yak and cattle GHs as compared to the other mammalian GHs. cGH carrying a His6-tag at the N-terminus was expressed in E. coli up to 50 % of the total cell protein. The expressed protein was in the form of inclusion bodies, which were solubilized with 6 M guanidinium hydrochloride and then purified by metal affinity chromatography. The purified cGH, refolded by a step-wise dialysis process, was over 92 % purified and this product was biologically active as determined by Nb2 rat lymphoma cell proliferation assay.