Gene expression pattern in rat bone marrow mesenchymal stem cells in response tohypoxia

Kanwal Haneef; Nadia Naeem; Hana'a Iqbal; Nurul Kabir; Asmat Salim; Siddiqua Jamall

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Gene expression pattern in rat bone marrow mesenchymal stem cells in response tohypoxia

Author(s)

Kanwal Haneef Dr. Panjwani Center for Molecular Medicine & Drug Research, International centre for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN

Nadia Naeem Dr. Panjwani Center for Molecular Medicine & Drug Research, International centre for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN

Hana'a Iqbal Dr. Panjwani Center for Molecular Medicine & Drug Research, International centre for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN

Nurul Kabir Dr. Panjwani Center for Molecular Medicine & Drug Research, International centre for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN

Asmat Salim Dr. Panjwani Center for Molecular Medicine & Drug Research, International centre for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN

Siddiqua Jamall Department of Biochemistry, University of Karachi, Karachi, PAKISTAN

Abstract

Under hypoxic stress condition, mesenchymal stem cells (MSCs) show an accelerated regeneration potential. This is called as “preconditioning” which results in the stimulation of endogenous mechanisms including protein expressions that protects against future lethal hypoxic insults. We have studied the effect of hypoxia on the expression of growth factors. Rat bone marrow-derived MSCs were cultured and were given hypoxia by optimized doses of 0.25mM and 0.5mM of 2, 4, dinitrophenol (DNP) at 10 and 20 min each and then allowed to propagate under normal condition for 24 hours. The morphological examination of MSCs has indicated that the cells were slightly shrunken immediately after the insults. Analysis of various cytokines through RT-PCR has shown higher expression of SCF, IL-7, TGFß, HIF, VEGF and HGF. The results indicated that MSCs secreted cell survival factors under hypoxic condition that operate via various signaling pathways enhancing their regenerating ability.

Publication Details

Page(s) 90-93

DOI DOI not available

Published Journal: Pakistan Journal of Biochemistry and Molecular Biology , Volume: 43, Issue: 2, Year: 2010

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