Characterizing the Amniotic Fluid-Derived Stem Cells and Optimizing the Passage Number for Targeted Applications

Tayfun Dikmen; Metin Erdoğan; Korhan Altunbaş

doi:10.29261/pakvetj/2024.226

Author(s)

Tayfun Dikmen University of Liverpool, Institute of Life Course and Medical Sciences, Dept. of Musculoskeletal and Aging Science, Liverpool, United Kingdom; Istanbul Health and Technology University, Faculty of Medicine, Dept. of Histology and Embryology, İstanbul, Türkiye

Metin Erdoğan Afyon Kocatepe University, Faculty of Veterinary Medicine, Dept. of Medical Biology and Genetics,Afyonkarahisar,Türkiye

Korhan Altunbaş Afyon Kocatepe University, Faculty of Veterinary Medicine, Dept. of Histology and Embryology,Afyonkarahisar,Türkiye

Abstract

Mesenchymal stem cells are considered potent sources that can be used for tissue regeneration and treatment of various diseases and syndromes. Among the mesenchymal stem cells, amniotic fluid-derived stem cells come forward as they possess some pluripotent properties and there are no serious ethical concerns upon their derivation. Therefore, gaining a better understanding of the nature of amniotic fluid stem cells is important. Although it is known that stem cells show slightly different characteristics between passages, scientists often consider only cell numbers and the proliferation pattern of the cells when deciding the passage to use in their studies. In this study, it was aimed to characterize rat amniotic fluid-derived stem cells for their mesenchymal and pluripotent features and make inter-passage comparisons by real-time qPCR to reveal their distinctions between different passages, and eventually help decide the appropriate passage numbers to be used in future research. The outcomes of the study showed that using rat amniotic fluid-derived stem cells at P5 could be beneficial for mesodermal differentiation studies and using them in earlier passages may be more favorable for the studies requiring better ectodermal differentiation properties.

Publication Details

Page(s) 854-860

DOI 10.29261/pakvetj/2024.226

Published Journal: Pakistan Veterinary Journal, Volume: 44, Issue: 3, Year: 2024

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Keywords

amniotic fluid amniotic fluid-derived stem cells mesenchymal pluripotent differentiation inter-passage difference.

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