Isolated bacterial strains efficiently degrade glyphosate under different environmental conditions

WAQAS MOHY-UD-DIN; MUHAMMAD JAVED AKHTAR; SAFDAR BASHIR; HAFIZ NAEEM ASGHAR; MUHAMMAD FARRAKH NAWAZ; FENG CHEN

doi:10.30848/PJB2024-2(28)

Author(s)

WAQAS MOHY-UD-DIN Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad , Pakistan; Department of soil and Environmental Sciences, Ghazi University, D. G. Khan , Pakistan;Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, United states of America

MUHAMMAD JAVED AKHTAR Institute of Soil and Environmental Sciences, University of Agriculture,Faisalabad 38040,Pakistan

SAFDAR BASHIR Department of soil and Environmental Sciences, Ghazi University,D. G. Khan, 32200,Pakistan

HAFIZ NAEEM ASGHAR Institute of Soil and Environmental Sciences, University of Agriculture,Faisalabad 38040,Pakistan

MUHAMMAD FARRAKH NAWAZ Department of Forestry & Range Management, University of Agriculture,Faisalabad 38040,Pakistan

FENG CHEN Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science,Baltimore, MD 21202, United states of America,USA

Abstract

Glyphosate (GLX) is a crucial organophosphonate herbicide applied to eliminate unwanted, grasses and herbaceous plants in various vegetation management situations. Its widespread and consequently application is causing environmental pollution, there is a need to remove it from the environment using an eco-friendly and cost-effective method. Biodegradation is an effective method for the removal of GLX. But various environmental factor effecting is degradation after its applications such as aerobic and anaerobic environment, different pH and temperature, soil water contents and soil textures. Glyphosate resistant bacterial strains have been isolated from GLX-contaminated soil which were proficient to utilize GLX as a carbon source. High Pressure Liquid Chromatography instrument was used to determine the rate of GLX biodegradation in liquid media and soil under different environmental conditions. The five out of eleven strains such as WAG2 (Serratia liquefaciens), WAG4 (Klebsiella variicola), WAG45 (Enterobacter cloacae), WAG9 (Pseudomonas aeruginosa), and WAG11 (Enterobacter ludwigii) were proficient in average degraded 95 to 98 mg kg-1 glyphosate in soil followed by liquid media 93 to 96 mg kg-1 within 28 days of incubation in different environmental factors. The optimal circumstances for the degradation of GLX were found to be aerobic, pH 7, 40°C, soil water content at 10% and sandy loam texture showed maximum degradation at 100 mg kg-1 compared to 200 mg kg-1 concentration of GLX. This study demonstrates the potential of isolated bacterial strains for efficient degradation of GLX, which can be exploited for remediation of GLX in contaminated soil.

Publication Details

Page(s) 765-774

DOI 10.30848/PJB2024-2(28)

Published Journal: Pakistan Journal of Botany, Volume: 56, Issue: 2, Year: 2024

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Keywords

herbicides, Rhizobacteria, bioremediation, Biodegradation, Organophosphate, Rhizobacteria

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