Fabrication, characterization, process investigation and optimization of experimental conditions for the production of Indomethacin nanocrystals using microfluidic channel reactor

Jahangir Khan; Attiqa Naz; Shujaat Ahmad; Nauman Rahim Khan; Abidullah; Kifayat Ullah Shah; Imran Tariq; Muhammad Asif Khan; Haya Hussain; Hamid Hussain Afridi; Hind Ahmad Siddiq

doi:10.36721/PJPS.2023.36.1.SP.265-272.1

Jahangir Khan Department of Pharmacy, University of Malakand, Malakand, Pakistan; Institute of Life Science Research, School of Pharmacy, University of Bradford, UK; Department of Chemistry, Lancaster University, LA1 4YB, UK

Attiqa Naz Department of Pharmacy, Abasyn University,Peshawar,Pakistan

Shujaat Ahmad Department of Pharmacy, Shaheed Benazir Bhutto University Sheringal,Dir Upper,Pakistan

Nauman Rahim Khan Department of Pharmacy, Kohat University of Science and Technology,Kohat,Pakistan

Abidullah Department of Pharmacy, Shaheed Benazir Bhutto University Sheringal,Dir Upper,Pakistan

Kifayat Ullah Shah Department of Pharmaceutics, Faculty of Pharmacy, Gomal University,DI Khan,Pakistan

Imran Tariq Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan

Muhammad Asif Khan Department of Pharmacy, Sarhad University of Science & IT,Peshawar,Pakistan

Haya Hussain Department of Pharmacy, Shaheed Benazir Bhutto University Sheringal,Dir Upper,Pakistan

Hamid Hussain Afridi Department of Pharmacy, Shaheed Benazir Bhutto University Sheringal,Dir Upper,Pakistan

Hind Ahmad Siddiq Department of Chemistry, Lancaster University, LA1 4YB, UK; Department of Chemistry, College of Science, Jazan University, Saudi Arabia

Indomethacin a Non-steroidal anti-inflammatory drug (NSAID) demonstrates low bioavailability. To counter the problem an attempt was made by preparing indomethacin nanocrystals in a micro-channel fluid reactor using different concentrations of the polymers. Indomethacin crystals were optimized by determining the particle size of 380 nm ± 5.0 with PDI of 0.29±0.05. The crystalline nature was confirmed with the help of Powder X-Ray diffraction and Differential scanning calorimetry. A high antisolvent volume (2.0/0.5ml/min), low inlet angle (10°) and mixing time of 60 min with 15 min sonication at a high rate of stirring were the key parameters that produced Indomethacin nanocrystals with good particles size. A high anti-solvent volume (2.0/0.5ml/min), low inlet angle (10°) and mixing time of 60 min with 15 min sonication at a high rate of stirring were the key parameters that produced Indomethacin nanocrystals with good particle size. Similarly, the polymeric combination of including Polyvinylpyrrolidone k-30 (1%)- Hydroxypropyl methyl cellulose-15cps (0.5%) and Sodium lauryl sulfate (0.5%) were the appropriate concentrations. The Transmission electron microscopy and Scanning electron microscopy studies demonstrated the homogeneity with a distinct crystalline structure. The stability study displayed stability at 4oC and 25oC. The nanocrystals exhibited a dissolution rate correlated to the marketed and unprocessed drugs.