Bacillus subtilis subsp. subtilis STGG 14 is isolated ricephyllosphere environment which are proved to have antifungal activity toward Pyricularia oryzae race 173. The antifungal genes owned by B. subtilis subsp. subtilis STGG 14 are assumed playing important role in interfering the growth of P. oryzae. Therefore, this research aimed to discover the antifungal gene from B. subtilis subsp. subtilis STGG 14 and analyse the properties of the crude extract. Antifungal genes from B. subtilis subsp. subtilis STGG 14 were amplified using PCR method with several primers which encode different antifungal compounds. In this study, we successfully amplified ytpA gene which encodes lysophospholipase like enzyme (lysophospholipase YtpA) that involved in bacilysocin synthesis. Furthermore, the intracellular crude phospholipid compounds of B. subtilis subsp. subtilis STGG 14 was obtained from bacterial culture in NG (nutrient growth) medium using butanol and methanol as solvent. The intracellular crude phospholipid compounds were then analysed using GC-MS (Gas Chromatography/Mass Spectrometry) and FTIR (FourierTransform Infrared Spectroscopy) to reveal its antifungal properties. Interestingly, methyl tetradecanoate, the fatty acid structure of bacilysocin have been detected using GC-MS. This finding is supported by FTIR analysis which revealed the existence of several functional group such as C=O, C-H3, P=O and P-O-C. In addition, 15 chemical properties from crude extract are also detected by GC/MS and 5 of them displayed antifungal activities according to several references. According to the results, the crude extract produced by Bacillus subtilis subsp. subtilis STGG 14 contained phospholipid compound, but further study is needed to prove the obtained phospholipid compounds are bacilysocin as the existence of ytpA gene.