Wistar Rat as Photoaging Mouse Model

Damayanti; Cita Rosita Sigit Prakoeswa; Djoko Agus Purwanto; Anang Endaryanto; Muhammad Yulianto Listiawan; Yohanes Widodo Wirohadidjoyo; Soetjipto; Siswandono; Budi Utomo

Damayanti Doctoral Study Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia. ; Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Indonesia.

Cita Rosita Sigit Prakoeswa Department of Dermatology and Venereology, Faculty of Medicine,Universitas Airlangga,Indonesia

Djoko Agus Purwanto Department of Pharmaceutical Chemistry, Faculty of Pharmacy,Universitas Airlangga,Indonesia

Anang Endaryanto Department of Pediatrics, Faculty of Medicine,Universitas Airlangga,Indonesia

Muhammad Yulianto Listiawan Department of Dermatology and Venereology, Faculty of Medicine,Universitas Airlangga,Indonesia

Yohanes Widodo Wirohadidjoyo Department of Dermatology and Venereology, Faculty of Medicine,Universitas Gajah Mada,Indonesia

Soetjipto Department of Biochemistry, Faculty of Medicine,Universitas Airlangga,Indonesia

Siswandono Department of Pharmaceutical Chemistry, Faculty of Pharmacy,Universitas Airlangga,Indonesia

Budi Utomo Department of Community Medicine, Faculty of Medicine,Universitas Airlangga,Indonesia

Background The increasing of human life expectancy may cause aging problems, including photoaging. Photoaging raises new problems in community, because its influence in human selfesteem and quality of life. Studies about photoaging have been extensively performed to enhance the basic mechanism of photoaging. Mouse skin is usually used as cutaneous aging model, because it is genetically similar to human skin. Methods This study was true experimental study, post test only-control group design that was aimed to evaluate the role of ultraviolet (UV) B irradiation to produce photoaging mouse model. Twenty-four Wistar rats were divided randomly allocation into 2 groups, namely non-UV irradiated group and UV irradiated group. Ultraviolet B irradiation were performed for 5 weeks until reaching the total dose 3100 mJ/cm2. Dermal collagen density and transepidermal water loss (TEWL) level were evaluated after the intervention. Results Dermal collagen density in non-UV irradiated group and UV irradiated group were 38.21±2.67% and 27.73±0.77%. TEWL level in non-UV irradiated group and UV irradiated group were 7.55±0.89 g/m2/h and 17.53±3.94 g/m2/h. There was significant difference between the groups in dermal collagen density and TEWL level (p<0.05). Conclusion Wistar rat in UV irradiated group that were given total dose 3100 mJ/cm2 of UV B for 5 weeks has been proven to become photoaging mouse model.