Polymer capped transition metal oxide nanoparticles (TMO-NPs) are becoming a major thrust of modern research due to potential application in antibacterial and anticancer activity. The polymer capping agents such as Citric acid, Malic acid are used to enhance the sensitivity and played the vital role to control the size, morphology and monodispersity of TMO-NPs. The present study will be related to synthesis of pure and capped Fe3O4 NPs by using chemical method. The prepared Iron oxide-NPs will be characterized by using various characterization techniques such as XRD, SEM, FTIR, VSM and in vitro and in vivo bioassay. The XRD will be preferred to identify the crystal structure and also used to calculate the crystallite size of pure and capped Fe3O4 -NPs. The SEM analysis will be used to investigate the surface morphology. The FTIR will be measured the different functional groups attached on the surface of NPs. Moreover, the VSM analysis will be performed to measure the magnetization power of pure and capped Fe3O4-NPs. At the end material related study will be provided the information about the Fe3O4-NPs is suitable for antibacterial and anticancer activity.