Aim: To assess the knowledge of nursing students regarding Basic Life Support (BLS) a Private School of Nursing in Lahore, Pakistan. Study deign: Cross sectional study design was used in this study. Place and duration of study: This study was conducted at one of the private School of Nursing in Lahore and during November, 2018 to April, 2019. Methodology: A total of 60 student nurses of BSN, Post RN, and MSN programs participated in the study; all were practically involved in patient's care (under-supervision) at different tertiary level care hospitals of Punjab, Pakistan. All hospitals are responsible to keep their nurses' knowledge of BLS updated. This study has assessed knowledge of student nurses and has observed the difference in knowledge according to their qualification and experiences. Twenty five questions were used to assess participants' knowledge of BLS. Results: The findings revealed that 32 (53.34%) student nurses were well aware of the BLS, 15 (25%) had moderate knowledge while 13 (21.67%) of respondents had inadequate knowledge. Interestingly, analysis showed that there was a clear difference in BLS knowledge and their level of qualification and experience. Conclusion: Nursing students found to have satisfactory level of knowledge of basic life support, however, number of years of experience and qualification plays a significant role in its implication.