Mesenchymal stem cell (MSCs) research has played a significant role in field of medicine and dentistry. MSCs of dental origin can be harvested from both deciduous and permanent tooth. These cells can be differentiated into other cell varieties, such as odontoblasts, osteoblasts, chondrocytes, adipocytes, endothelial, neuronal and melanocytes making it a perfect cell source for regeneration of various tissues. These magical cells can be used to treat Parkinson's disease, Myocardial infarction, and Alzheimer's disease etc. It can also provide an attractive solution for hair, bone or tooth loss as well. Methods: We systematically searched and reviewed articles until July 2020, available on PubMed, Google Scholar and WHO database. Key word used to search the required topic was "dental pulp stem cells", "differentiation potential," "cryopreservation". Suitable full-text articles were selected and data were extracted. Conclusion: This review defines innovative discoveries in the area of dental pulp stem cell research and their possible application in the tissue regeneration.