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Inter-simple sequence repeat markers variation among natural accessions of moroccan carob tree (Ceratonia siliqua).

Author(s)
Ibrahim Konate University of Abobo -Adjame, URES - Daloa, Côte d`Ivoire, Daloa, R. Cote d`Ivoire, Morocco
El Bekkay Berraho Faculty of Sciences, Laboratory of Microbiology and Molecular Biology (LMBM), University Mohammed V-Agdal, Rabat, Morocco
Abdelkarim Filali-Maltouf Faculty of Sciences, Laboratory of Microbiology and Molecular Biology (LMBM), University Mohammed V-Agdal, Rabat, Morocco
Abstract
We adapt an efficient maxi-preparation for the extraction of bulk DNA from leaves of the carob tree (Ceratonia siliqua). The bulk DNA quality and quantity were suitable for molecular analysis. Ten Moroccan accessions of carob were evaluated for molecular diversity by using ISSR markers. 176 reproducible amplification fragments were generated with a total of 16 primers. 77.27% of bands were polymorphic. A high genetic diversity values were obtained with all the primers, except for primer F3, with an average of 0.57. Fourteen primers produced some 40 monomorphic bands which can be considered to be specific to the carob accessions studied. The clustering of the different accessions showed a tight relationship with their origin distribution. ISSR technology also proved useful in discrimination closely related natural carob accessions.
Publication Details
Page(s) 168-172
DOI DOI not available
Published Journal: International Journal of Agriculture and Biology, Volume: 11, Issue: 2, Year: 2009

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