Over expression of a synthetic gene encoding interferon lambda using relative synonymous codon usage bias in Escherichia coli.

Hashaam  Akhtar; Samar  Akhtar; Syed Umer  Jan; Azka  Khan; Najam us Sahar Sadaf  Zaidi; Ishtiaq  Qadri

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Over expression of a synthetic gene encoding interferon lambda using relative synonymous codon usage bias in Escherichia coli.

Author(s)

Hashaam Akhtar Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan

Samar Akhtar Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan

Syed Umer Jan Department of Pharmacy, University of Balochistan, Quetta, Balochistan, Pakistan

Azka Khan Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan

Najam us Sahar Sadaf Zaidi Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan

Ishtiaq Qadri Head Medical Biotechnology, King Fahd Medical Research Center, King Abdul Aziz University, Jeddah, Saudi Arabia

Abstract

Interferon Lambda (IFN-λ) is a type III interferon which belongs to a novel family of cytokines and possesses antiviral and antitumor properties. It is unique in its own class of cytokines; because of the specificity towards its heterodimer receptors and its structural similarities with cytokines of other classes. This renders IFN-λ a better choice for the treatment against many diseases including viral hepatitis and human coronavirus (HCoV-EMC). The present study describes a computational approach known as relative synonymous codon usage (RSCU); used to enhance the expression of IFN-λ protein in a eukaryotic expression system. Manually designed and commercially synthesized IFN-λ gene was cloned into pET-22b expression plasmid under the control of inducible T7-lac promoter. Maximum levels of IFN-λexpression was observed with 0.4 mM IPTG in transformed E. coli incubated for 4 hours in LB medium. Higher concentrations of IPTG had no or negative effect on the expression of IFN-λ. This synthetically over expressed IFN-λcan be tested as a targeted treatment option for viral hepatitis after purification.

Publication Details

Page(s) 1181-1188

DOI DOI not available

Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 26, Issue: 6, Year: 2013

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