METHOD DEVELOPMENT, VALIDATION AND CALCULATION OF UNCERTAINTY FOR THE DETERMINATION OF LAMBDA- CYHALOTHRIN FROM COMMERCIAL FORMULATIONS THROUGH REVERSE-PHASE LIQUID CHROMATOGRAPHIC APPROACH

Zeshan Aslam; Muhammad Aslam Avais; Muhammad Rashid Farooq; Muhammad Adnan Rafique; Muhammad Zia Ul Haq; Arslan Nazarat; Ali Afzal; Muhammad Awais Khalid

Lambda-cyhalothrin is a pyrethroid insecticide posing an implicit curb on the pest of different crops especially cotton. The focus of the present study was the development and validation of a test procedure for the quantitative measurement of lambda-cyhalothrin from its different formulations and calculation of uncertainty. The objective of this validation method was to demonstrate its reliability for the desired application. This study was conducted at Pesticide Quality Control Laboratory, Kala Shah Kaku, Pakistan during 2019. Lambda-cyhalothrin was quantified by liquid chromatography with a diode array detector (DAD). The estimation was evaluated on agilent high performance liquid chromatograph (HPLC) equipped with C18 column using acetonitrile and water (85:15) as mobile phase. different steps of method validation, i.e., accuracy, precision, linearity, recovery percentage, the limit of detection (LOQ) and quantification (LOD) were performed and uncertainty was calculated according to the instruction on the validation of an analytical test method. The obtained data showed that the test method was accurate (99.25% accuracy), reliable (%RSD of repeatability 0.379), precise (%RSD of reproducibility 0.30), and high recovery percentage, i.e., 102.7%. The LOQ and LOD of this test method was 0.002% and 0.01%, respectively. The uncertainty value of this test method for the determination of lambda-cyhalothrin (2.70%) from its formulation was observed that was, 0.016%w/w with a 5% probability level. The findings of the study validated that the method were substantiated and all the observations fall within the appropriate limit. The developed method was considerably adoptive due to its simplicity, reliability, and practicality. Hence, the proposed method had the potential to be adopted for routine analysis with ease and convenience.